1aro
From Proteopedia
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| - | [[Image:1aro.jpg|left|200px]] | + | [[Image:1aro.jpg|left|200px]] |
| - | + | ||
| - | '''T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME''' | + | {{Structure |
| + | |PDB= 1aro |SIZE=350|CAPTION= <scene name='initialview01'>1aro</scene>, resolution 2.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HG:MERCURY (II) ION'>HG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | ||
| + | |GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T7]) | ||
| + | }} | ||
| + | |||
| + | '''T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ARO is a [ | + | 1ARO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARO OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme., Jeruzalmi D, Steitz TA, EMBO J. 1998 Jul 15;17(14):4101-13. PMID:[http:// | + | Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme., Jeruzalmi D, Steitz TA, EMBO J. 1998 Jul 15;17(14):4101-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9670025 9670025] |
[[Category: Bacteriophage t7]] | [[Category: Bacteriophage t7]] | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:01:30 2008'' |
Revision as of 08:01, 20 March 2008
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| , resolution 2.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | T7 (Bacteriophage T7) | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME
Overview
The T7 RNA polymerase-T7 lysozyme complex regulates phage gene expression during infection of Escherichia coli. The 2.8 A crystal structure of the complex reveals that lysozyme binds at a site remote from the polymerase active site, suggesting an indirect mechanism of inhibition. Comparison of the T7 RNA polymerase structure with that of the homologous pol I family of DNA polymerases reveals identities in the catalytic site but also differences specific to RNA polymerase function. The structure of T7 RNA polymerase presented here differs significantly from a previously published structure. Sequence similarities between phage RNA polymerases and those from mitochondria and chloroplasts, when interpreted in the context of our revised model of T7 RNA polymerase, suggest a conserved fold.
About this Structure
1ARO is a Protein complex structure of sequences from Bacteriophage t7. Full crystallographic information is available from OCA.
Reference
Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme., Jeruzalmi D, Steitz TA, EMBO J. 1998 Jul 15;17(14):4101-13. PMID:9670025
Page seeded by OCA on Thu Mar 20 10:01:30 2008
