3rj8

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[[Image:3rj8.jpg|left|200px]]
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==Crystal structure of carbohydrate oxidase from Microdochium nivale==
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<StructureSection load='3rj8' size='340' side='right' caption='[[3rj8]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3rj8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Microdochium_nivale Microdochium nivale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RJ8 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rja|3rja]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MnCO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5520 Microdochium nivale])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose_oxidase Glucose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.4 1.1.3.4] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rj8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rj8 RCSB], [http://www.ebi.ac.uk/pdbsum/3rj8 PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microdochium nivale carbohydrate oxidase was produced by heterologous recombinant expression in Aspergillus oryzae, purified and crystallized. The enzyme crystallizes with varying crystal morphologies depending on the crystallization conditions. Several different crystal forms were obtained using the hanging-drop vapour-diffusion method, two of which were used for diffraction measurements. Hexagon-shaped crystals (form I) diffracted to 2.66 A resolution, with unit-cell parameters a = b = 55.7, c = 610.4 A and apparent space group P6(2)22. Analysis of the data quality showed almost perfect twinning of the crystals. Attempts to solve the structure by molecular replacement did not give satisfactory results. Recently, clusters of rod-shaped crystals (form II) were grown in a solution containing PEG MME 550. These crystals belonged to the monoclinic system C2, with unit-cell parameters a = 132.9, b = 56.6, c = 86.5 A, beta = 95.7 degrees . Data sets were collected to a resolution of 2.4 A. The structure was solved by the molecular-replacement method. Model refinement is currently in progress.
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Crystallization of carbohydrate oxidase from Microdochium nivale.,Duskova J, Dohnalek J, Skalova T, Ostergaard LH, Fuglsang CC, Kolenko P, Stepankova A, Hasek J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt 6):638-40., Epub 2009 May 23. PMID:19478452<ref>PMID:19478452</ref>
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The line below this paragraph, containing "STRUCTURE_3rj8", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3rj8| PDB=3rj8 | SCENE= }}
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===Crystal structure of carbohydrate oxidase from Microdochium nivale===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_19478452}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 19478452 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_19478452}}
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==About this Structure==
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[[3rj8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Microdochium_nivale Microdochium nivale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RJ8 OCA].
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==Reference==
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<ref group="xtra">PMID:019478452</ref><references group="xtra"/>
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[[Category: Glucose oxidase]]
[[Category: Glucose oxidase]]
[[Category: Microdochium nivale]]
[[Category: Microdochium nivale]]

Revision as of 04:47, 5 June 2014

Crystal structure of carbohydrate oxidase from Microdochium nivale

3rj8, resolution 2.40Å

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