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3r6w

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[[Image:3r6w.png|left|200px]]
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==paAzoR1 binding to nitrofurazone==
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<StructureSection load='3r6w' size='340' side='right' caption='[[3r6w]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3r6w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R6W FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NFZ:NITROFURAZONE'>NFZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2v9c|2v9c]], [[3lt5|3lt5]], [[3keg|3keg]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">azoR1, PA0785 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r6w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r6w RCSB], [http://www.ebi.ac.uk/pdbsum/3r6w PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Azoreductases are well known for azo pro-drug activation by gut flora. We show that azoreductases have a wider role in drug metabolism than previously thought as they can also reduce and hence activate nitrofurazone. Nitrofurazone, a nitroaromatic drug, is a broad spectrum antibiotic which has until now been considered as activated in bacteria by nitroreductases. The structure of the azoreductase with nitrofurazone bound was solved at 2.08 A and shows nitrofurazone in an active conformation. Based on the structural information, the kinetics and stoichiometry of nitrofurazone reduction by azoreductase from P. aeruginosa, we propose a mechanism of activation which accounts for the ability of azoreductases to reduce both azo and nitroaromatic drugs. This mode of activation can explain the cytotoxic side-effects of nitrofurazone through human azoreductase homologues.
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Activation of nitrofurazone by azoreductases: multiple activities in one enzyme.,Ryan A, Kaplan E, Laurieri N, Lowe E, Sim E Sci Rep. 2011;1:63. Epub 2011 Aug 12. PMID:22355582<ref>PMID:22355582</ref>
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The line below this paragraph, containing "STRUCTURE_3r6w", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3r6w| PDB=3r6w | SCENE= }}
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===paAzoR1 binding to nitrofurazone===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22355582}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 22355582 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_22355582}}
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==About this Structure==
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[[3r6w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R6W OCA].
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==Reference==
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<ref group="xtra">PMID:022355582</ref><references group="xtra"/>
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Kaplan, K.]]
[[Category: Kaplan, K.]]

Revision as of 04:52, 5 June 2014

paAzoR1 binding to nitrofurazone

3r6w, resolution 2.08Å

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