3rse

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[[Image:3rse.png|left|200px]]
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==Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex==
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<StructureSection load='3rse' size='340' side='right' caption='[[3rse]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3rse]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RSE FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rse RCSB], [http://www.ebi.ac.uk/pdbsum/3rse PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Actin-related protein (Arp) 2/3 complex mediates the formation of actin filament branches during endocytosis and at the leading edge of motile cells. The pathway of branch formation is ambiguous owing to uncertainty regarding the stoichiometry and location of VCA binding sites on Arp2/3 complex. Isothermal titration calorimetry showed that the CA motif from the C terminus of fission yeast WASP (Wsp1p) bound to fission yeast and bovine Arp2/3 complex with a stoichiometry of 2 to 1 and very different affinities for the two sites (K(d)s of 0.13 and 1.6 muM for fission yeast Arp2/3 complex). Equilibrium binding, kinetic, and cross-linking experiments showed that (i) CA at high-affinity site 1 inhibited Arp2/3 complex binding to actin filaments, (ii) low-affinity site 2 had a higher affinity for CA when Arp2/3 complex was bound to actin filaments, and (iii) Arp2/3 complex had a much higher affinity for free CA than VCA cross-linked to an actin monomer. Crystal structures showed the C terminus of CA bound to the low-affinity site 2 on Arp3 of bovine Arp2/3 complex. The C helix is likely to bind to the barbed end groove of Arp3 in a position for VCA to deliver the first actin subunit to the daughter filament.
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Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.,Ti SC, Jurgenson CT, Nolen BJ, Pollard TD Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):E463-71. Epub 2011 Jun 15. PMID:21676862<ref>PMID:21676862</ref>
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The line below this paragraph, containing "STRUCTURE_3rse", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_3rse| PDB=3rse | SCENE= }}
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===Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21676862}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21676862 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21676862}}
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==About this Structure==
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[[3rse]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RSE OCA].
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==Reference==
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<ref group="xtra">PMID:021676862</ref><references group="xtra"/>
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Jurgenson, C T.]]
[[Category: Jurgenson, C T.]]

Revision as of 04:52, 5 June 2014

Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex

3rse, resolution 2.65Å

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