3qrx

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[[Image:3qrx.png|left|200px]]
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==Chlamydomonas reinhardtii centrin bound to melittin==
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<StructureSection load='3qrx' size='340' side='right' caption='[[3qrx]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3qrx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QRX FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VFL2, CHLREDRAFT_159554 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qrx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qrx RCSB], [http://www.ebi.ac.uk/pdbsum/3qrx PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Centrin is a calcium binding protein (CaBP) belonging to the EF-hand superfamily. As with other proteins within this family, centrin is a calcium sensor with multiple biological target proteins. We chose to study Chlamydomonas reinhardtii centrin (Crcen) and its interaction with melittin (MLT) as a model for CaBP complexes due to its amphipathic properties. Our goal was to determine the molecular interactions that lead to centrin-MLT complex formation, their relative stability, and the conformational changes associated with the interaction, when compared to the single components. For this, we determined the thermodynamic parameters that define Crcen-MLT complex formation. Two-dimensional infrared (2D IR) correlation spectroscopy were used to study the amide I', I'*, and side chain bands for (13) C-Crcen, MLT, and the (13) C-Crcen-MLT complex. This approach resulted in the determination of MLT's increased helicity, while centrin was stabilized within the complex. Herein we provide the first complete molecular description of centrin-MLT complex formation and the dissociation process. Also, discussed is the first structure of a CaBP-MLT complex by X-ray crystallography, which shows that MLT has a different binding orientation than previously characterized centrin-bound peptides. Finally, all of the experimental results presented herein are consistent with centrin maintaining an extended conformation while interacting with MLT. The molecular implications of these results are: (1) the recognition of hydrophobic contacts as requirements for initial binding, (2) minimum electrostatic interactions within the C-terminal end of the peptide, and (3) van der Waals interactions within MLTs N-terminal end are required for complex formation. Proteins 2011; (c) 2011 Wiley-Liss, Inc.
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The structure, molecular dynamics, and energetics of centrin-melittin complex.,Sosa Ldel V, Alfaro E, Santiago J, Narvaez D, Rosado MC, Rodriguez A, Gomez AM, Schreiter ER, Pastrana-Rios B Proteins. 2011 Nov;79(11):3132-43. doi: 10.1002/prot.23142. Epub 2011 Aug, 30. PMID:21989934<ref>PMID:21989934</ref>
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The line below this paragraph, containing "STRUCTURE_3qrx", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3qrx| PDB=3qrx | SCENE= }}
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===Chlamydomonas reinhardtii centrin bound to melittin===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21989934}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21989934 is the PubMed ID number.
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</StructureSection>
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[[Category: Chlre]]
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{{ABSTRACT_PUBMED_21989934}}
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==About this Structure==
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[[3qrx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRX OCA].
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==Reference==
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<ref group="xtra">PMID:021989934</ref><references group="xtra"/>
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[[Category: Chlamydomonas reinhardtii]]
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[[Category: Schreiter, E R.]]
[[Category: Schreiter, E R.]]
[[Category: Calcium binding]]
[[Category: Calcium binding]]

Revision as of 04:55, 5 June 2014

Chlamydomonas reinhardtii centrin bound to melittin

3qrx, resolution 2.20Å

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