3sdw

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[[Image:3sdw.png|left|200px]]
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==Crystal structure of a ribose-5-phosphate isomerase B RpiB from Coccidioides immitis bound to phosphate==
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<StructureSection load='3sdw' size='340' side='right' caption='[[3sdw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3sdw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SDW FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qd5|3qd5]], [[3s5p|3s5p]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CIMG_07932 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5501 Coccidioides immitis])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sdw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sdw RCSB], [http://www.ebi.ac.uk/pdbsum/3sdw PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ABSTRACT: BACKGROUND: Ribose-5-phosphate isomerase is an enzyme that catalyzes the interconversion of ribose-5-phosphate and ribulose-5-phosphate. This family of enzymes naturally occurs in two distinct classes, RpiA and RpiB, which play an important role in the pentose phosphate pathway and nucleotide and co-factor biogenesis. RESULTS: Although RpiB occurs predominantly in bacteria, here we report crystal structures of a putative RpiB from the pathogenic fungus Coccidioides immitis. A 1.9 A resolution apo structure was solved by combined molecular replacement and single wavelength anomalous dispersion (SAD) phasing using a crystal soaked briefly in a solution containing a high concentration of iodide ions. RpiB from C. immitis contains modest sequence and high structural homology to other known RpiB structures. A 1.8 A resolution phosphate-bound structure demonstrates phosphate recognition and charge stabilization by a single positively charged residue whereas other members of this family use up to five positively charged residues to contact the phosphate of ribose-5-phosphate. A 1.7 A resolution structure was obtained in which the catalytic base of C. immitis RpiB, Cys76, appears to form a weakly covalent bond with the central carbon of malonic acid with a bond distance of 2.2 A. This interaction may mimic that formed by the suicide inhibitor iodoacetic acid with RpiB. CONCLUSION: The C. immitis RpiB contains the same fold and similar features as other members of this class of enzymes such as a highly reactive active site cysteine residue, but utilizes a divergent phosphate recognition strategy and may recognize a different substrate altogether.
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Structural characterization of a ribose-5-phosphate isomerase B from the pathogenic fungus Coccidioides immitis.,Edwards TE, Abramov AB, Smith ER, Baydo RO, Leonard JT, Leibly DJ, Thompkins KB, Clifton MC, Gardberg AS, Staker BL, Van Voorhis WC, Myler PJ, Stewart LJ BMC Struct Biol. 2011 Oct 13;11(1):39. PMID:21995815<ref>PMID:21995815</ref>
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The line below this paragraph, containing "STRUCTURE_3sdw", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_3sdw| PDB=3sdw | SCENE= }}
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===Crystal structure of a ribose-5-phosphate isomerase B RpiB from Coccidioides immitis bound to phosphate===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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*[[Ribose-5-phosphate isomerase|Ribose-5-phosphate isomerase]]
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The line below this paragraph, {{ABSTRACT_PUBMED_21995815}}, adds the Publication Abstract to the page
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== References ==
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(as it appears on PubMed at http://www.pubmed.gov), where 21995815 is the PubMed ID number.
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<references/>
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__TOC__
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{{ABSTRACT_PUBMED_21995815}}
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</StructureSection>
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==About this Structure==
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[[3sdw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDW OCA].
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==Reference==
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<ref group="xtra">PMID:021995815</ref><references group="xtra"/>
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[[Category: Coccidioides immitis]]
[[Category: Coccidioides immitis]]
[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]
[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]

Revision as of 05:27, 5 June 2014

Crystal structure of a ribose-5-phosphate isomerase B RpiB from Coccidioides immitis bound to phosphate

3sdw, resolution 1.80Å

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