3s4g

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[[Image:3s4g.png|left|200px]]
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==Low Resolution Structure of STNV complexed with RNA==
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<StructureSection load='3s4g' size='340' side='right' caption='[[3s4g]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3s4g]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Tobacco_necrosis_satellite_virus_1 Tobacco necrosis satellite virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S4G FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rqv|3rqv]], [[1vtz|1vtz]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s4g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s4g RCSB], [http://www.ebi.ac.uk/pdbsum/3s4g PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A codon-optimised gene has been expressed in Escherichia coli to produce the coat protein (CP) of the Satellite Tobacco Necrosis Virus. This protein assembles in vivo into capsids closely resembling those of the T=1 wild-type virus. These virus-like particles (VLPs) package the recombinant mRNA transcript and can be disassembled and reassembled using different buffer conditions. The X-ray crystal structure of the VLP has been solved and refined at 1.4 A resolution and shown to be very similar to that of wild-type Satellite Tobacco Necrosis Virus, except that icosahedral symmetry constraints could be removed to reveal differences between subunits, presumably owing to crystal packing. An additional low-resolution X-ray crystal structure determination revealed well-ordered RNA fragments lodged near the inside surface of the capsid, close to basic clusters formed by the N-terminal helices that project into the interior of the particle. The RNA consists of multiple copies of a 3-bp helical stem, with a single unpaired base at the 3' end, and probably consists of a number of short stem-loops where the loop region is disordered. The arrangement of the RNA is different from that observed in other satellite viruses.
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Construction and Crystal Structure of Recombinant STNV Capsids.,Lane SW, Dennis CA, Lane CL, Trinh CH, Rizkallah PJ, Stockley PG, Phillips SE J Mol Biol. 2011 Oct 14;413(1):41-50. Epub 2011 Aug 3. PMID:21839089<ref>PMID:21839089</ref>
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The line below this paragraph, containing "STRUCTURE_3s4g", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3s4g| PDB=3s4g | SCENE= }}
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===Low Resolution Structure of STNV complexed with RNA===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21839089}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21839089 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21839089}}
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==About this Structure==
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[[3s4g]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Tobacco_necrosis_satellite_virus_1 Tobacco necrosis satellite virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S4G OCA].
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==Reference==
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<ref group="xtra">PMID:021839089</ref><references group="xtra"/>
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[[Category: Tobacco necrosis satellite virus 1]]
[[Category: Tobacco necrosis satellite virus 1]]
[[Category: Dennis, C A.]]
[[Category: Dennis, C A.]]

Revision as of 05:29, 5 June 2014

Low Resolution Structure of STNV complexed with RNA

3s4g, resolution 6.00Å

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