3tsi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3tsi.png|left|200px]]
+
==Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain==
 +
<StructureSection load='3tsi' size='340' side='right' caption='[[3tsi]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3tsi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Parainfluenza_virus_5 Parainfluenza virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TSI FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11207 Parainfluenza virus 5])</td></tr>
 +
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tsi RCSB], [http://www.ebi.ac.uk/pdbsum/3tsi PDBsum]</span></td></tr>
 +
<table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Paramyxovirus entry into cells requires the fusion protein (F) and a receptor binding protein (HN, H or G). The multifunctional hemagglutinin-neuraminidase (HN) protein of some paramyxoviruses, besides functioning as the receptor (sialic acid) binding protein (hemagglutinin activity) and the receptor-destroying protein (neuraminidase activity), enhances fusion protein (F) activity, presumably by lowering the activation energy required for F to mediate fusion of viral and cellular membranes. Before or upon receptor binding by the HN globular head, F is believed to interact with the HN stalk. Unfortunately until recently none of the receptor binding protein crystal structures have shown electron density for the stalk domain. Parainfluenza virus 5 (PIV5) HN exists as a non-covalent dimer-of-dimers on the surface of cells, linked by a single disulfide bond in the stalk. Here we present the crystal structure of the PIV5-HN stalk domain at a resolution of 2.65 A revealing a four-helix bundle (4HB) with an upper (N-terminal) straight region and a lower (C-terminal) supercoiled part. The hydrophobic core residues are a mix of an 11-mer repeat and a 3-4 heptad repeat. To functionally characterize the role of the HN stalk in F-interactions and fusion, we designed mutants along the PIV5-HN stalk that are N-glycosylated to physically disrupt F-HN interactions. By extensive study of receptor binding, neuraminidase activity, oligomerization and fusion promoting functions of the mutant proteins we found a correlation between the position of the N-glycosylation mutants on the stalk structure and their neuraminidase activities as well as their abilities to promote fusion.
-
<!--
+
Structure and mutagenesis of the parainfluenza virus 5 hemagglutinin-neuraminidase stalk domain reveals a four-helix bundle and the role of the stalk in fusion promotion.,Bose S, Welch BD, Kors CA, Yuan P, Jardetzky TS, Lamb RA J Virol. 2011 Oct 12. PMID:21994464<ref>PMID:21994464</ref>
-
The line below this paragraph, containing "STRUCTURE_3tsi", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_3tsi| PDB=3tsi | SCENE= }}
+
-
===Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain===
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
 +
</div>
-
 
+
==See Also==
-
<!--
+
*[[Hemagglutinin|Hemagglutinin]]
-
The line below this paragraph, {{ABSTRACT_PUBMED_21994464}}, adds the Publication Abstract to the page
+
== References ==
-
(as it appears on PubMed at http://www.pubmed.gov), where 21994464 is the PubMed ID number.
+
<references/>
-
-->
+
__TOC__
-
{{ABSTRACT_PUBMED_21994464}}
+
</StructureSection>
-
 
+
-
==About this Structure==
+
-
[[3tsi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Parainfluenza_virus_5 Parainfluenza virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSI OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:021994464</ref><references group="xtra"/>
+
[[Category: Exo-alpha-sialidase]]
[[Category: Exo-alpha-sialidase]]
[[Category: Parainfluenza virus 5]]
[[Category: Parainfluenza virus 5]]

Revision as of 05:47, 5 June 2014

Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain

3tsi, resolution 2.65Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3tsi"
Views
Personal tools
Navigation
Toolbox