2c7a

From Proteopedia

Revision as of 14:55, 30 October 2007

STRUCTURE OF THE PROGESTERONE RECEPTOR-DNA COMPLEX

Overview

The DNA binding domain (DBD) of nuclear hormone receptors contains a, highly conserved globular domain and a less conserved carboxyl-terminal, extension (CTE). Despite previous observations that the CTEs of some, classes of nuclear receptors are structured and interact with DNA outside, of the hexanucleotide hormone response element (HRE), there has been no, evidence for such a CTE among the steroid receptors. We have determined, the structure of the progesterone receptor (PR)-DBD-CTE DNA complex at a, resolution of 2.5 A, which revealed binding of the CTE to the minor groove, flanking the HREs. Alanine substitutions of the interacting CTE residues, reduced affinity for inverted repeat HREs separated by three nucleotides, and essentially abrogated binding to a single HRE. A highly ... [(full description)]

About this Structure

2C7A is a [Single protein] structure of sequence from [Homo sapiens] with ZN as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structure of the progesterone receptor-deoxyribonucleic acid complex: novel interactions required for binding to half-site response elements., Roemer SC, Donham DC, Sherman L, Pon VH, Edwards DP, Churchill ME, Mol Endocrinol. 2006 Dec;20(12):3042-52. Epub 2006 Aug 24. PMID:16931575

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