2c7f

From Proteopedia

Revision as of 14:55, 30 October 2007

THE STRUCTURE OF A FAMILY 51 ARABINOFURANOSIDASE, ARAF51, FROM CLOSTRIDIUM THERMOCELLUM IN COMPLEX WITH 1,5-ALPHA-L-ARABINOTRIOSE.

Overview

The digestion of the plant cell wall requires the concerted action of a, diverse repertoire of enzyme activities. An important component of these, hydrolase consortia are arabinofuranosidases, which release, L-arabinofuranose moieties from a range of plant structural, polysaccharides. The anaerobic bacterium Clostridium thermocellum, a, highly efficient plant cell wall degrader, possesses a single, alpha-L-arabinofuranosidase (EC 3.2.1.55), CtAraf51A, located in GH51, (glycoside hydrolase family 51). The crystal structure of the enzyme has, been solved in native form and in 'Michaelis' complexes with both, alpha-1,5-linked arabinotriose and alpha-1,3 arabinoxylobiose, both, forming a hexamer in the asymmetric unit. Kinetic studies reveal that, CtAraf51A, in contrast with ... [(full description)]

About this Structure

2C7F is a [Single protein] structure of sequence from [Clostridium thermocellum] with EDO as [ligand]. Active as [Alpha-N-arabinofuranosidase], with EC number [3.2.1.55]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum., Taylor EJ, Smith NL, Turkenburg JP, D'Souza S, Gilbert HJ, Davies GJ, Biochem J. 2006 Apr 1;395(1):31-7. PMID:16336192

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