1b3a
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1b3a.gif|left|200px]] | + | [[Image:1b3a.gif|left|200px]] |
| - | + | ||
| - | '''TOTAL CHEMICAL SYNTHESIS AND HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE POTENT ANTI-HIV PROTEIN AOP-RANTES''' | + | {{Structure |
| + | |PDB= 1b3a |SIZE=350|CAPTION= <scene name='initialview01'>1b3a</scene>, resolution 1.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=AOP:PENTYLOXYAMINO-ACETALDEHYDE'>AOP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''TOTAL CHEMICAL SYNTHESIS AND HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE POTENT ANTI-HIV PROTEIN AOP-RANTES''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1B3A is a [ | + | 1B3A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3A OCA]. |
==Reference== | ==Reference== | ||
| - | Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES., Wilken J, Hoover D, Thompson DA, Barlow PN, McSparron H, Picard L, Wlodawer A, Lubkowski J, Kent SB, Chem Biol. 1999 Jan;6(1):43-51. PMID:[http:// | + | Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES., Wilken J, Hoover D, Thompson DA, Barlow PN, McSparron H, Picard L, Wlodawer A, Lubkowski J, Kent SB, Chem Biol. 1999 Jan;6(1):43-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9889151 9889151] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barlow, P N.]] | [[Category: Barlow, P N.]] | ||
| Line 30: | Line 39: | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: hiv-1]] | [[Category: hiv-1]] | ||
| - | [[Category: | + | [[Category: rante]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:05:39 2008'' |
Revision as of 08:05, 20 March 2008
| |||||||
| , resolution 1.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TOTAL CHEMICAL SYNTHESIS AND HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE POTENT ANTI-HIV PROTEIN AOP-RANTES
Contents |
Overview
BACKGROUND: RANTES is a CC-type chemokine protein that acts as a chemoattractant for several kinds of leukocytes, playing an important pro-inflammatory role. Entry of human immunodeficiency virus-1 (HIV-1) into cells depends on the chemokine receptor CCR5. RANTES binds CCR5 and inhibits HIV-1 entry into peripheral blood cells. Interaction with chemokine receptors involves a distinct set of residues at the amino terminus of RANTES. This finding was utilized in the development of a chemically modified aminooxypentane derivative of RANTES, AOP-RANTES, that was originally produced from the recombinant protein using semisynthetic methods. RESULTS: AOP-RANTES has been produced by a novel total chemical synthesis that provides efficient, direct access to large amounts of this anti-HIV protein analog. The crystal structure of chemically synthesized AOP-RANTES has been solved and refined at 1.6 A resolution. The protein is a dimer, with the amino-terminal pentane oxime moiety clearly defined. CONCLUSIONS: Total chemical synthesis of AOP-RANTES provides a convenient method of producing the multi-milligram quantities of this protein needed to investigate the molecular basis of receptor binding and antiviral activity. This work provides the first truly high-resolution structure of a RANTES protein, although the structure of RANTES was known from previous nuclear magnetic resonance (NMR) determinations.
Disease
Known diseases associated with this structure: HIV-1 disease, delayed progression of OMIM:[187011], HIV-1 disease, rapid progression of OMIM:[187011]
About this Structure
1B3A is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES., Wilken J, Hoover D, Thompson DA, Barlow PN, McSparron H, Picard L, Wlodawer A, Lubkowski J, Kent SB, Chem Biol. 1999 Jan;6(1):43-51. PMID:9889151
Page seeded by OCA on Thu Mar 20 10:05:39 2008
Categories: Single protein | Barlow, P N. | Hoover, D. | Kent, S B.H. | Lubkowski, J. | Mcsparron, H. | Picard, L. | Thompson, D A. | Wilken, J. | Wlodawer, A. | AOP | SO4 | Chemical protein synthesis | Chemokine | Crystal structure | Hiv-1 | Rante
