3uso

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[[Image:3uso.png|left|200px]]
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==Crystal structure of LeuT bound to L-selenomethionine in space group P21212 from lipid bicelles==
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<StructureSection load='3uso' size='340' side='right' caption='[[3uso]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3uso]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3USO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3USO FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3usg|3usg]], [[3usi|3usi]], [[3usj|3usj]], [[3usk|3usk]], [[3usl|3usl]], [[3usm|3usm]], [[3usp|3usp]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">snf, aq_2077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uso OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uso RCSB], [http://www.ebi.ac.uk/pdbsum/3uso PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Neurotransmitter sodium symporters (NSSs) catalyze the uptake of neurotransmitters into cells, terminating neurotransmission at chemical synapses. Consistent with the role of NSSs in the central nervous system, they are implicated in multiple diseases and disorders. LeuT, from Aquifex aeolicus, is a prokaryotic ortholog of the NSS family and has contributed to our understanding of the structure, mechanism and pharmacology of NSSs. At present, however, the functional state of LeuT in crystals grown in the presence of n-octyl-beta-D-glucopyranoside (beta-OG) and the number of substrate binding sites are controversial issues. Here we present crystal structures of LeuT grown in DMPC-CHAPSO bicelles and demonstrate that the conformations of LeuT-substrate complexes in lipid bicelles and in beta-OG detergent micelles are nearly identical. Furthermore, using crystals grown in bicelles and the substrate leucine or the substrate analog selenomethionine, we find only a single substrate molecule in the primary binding site.
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Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context.,Wang H, Elferich J, Gouaux E Nat Struct Mol Biol. 2012 Jan 15;19(2):212-9. doi: 10.1038/nsmb.2215. PMID:22245965<ref>PMID:22245965</ref>
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The line below this paragraph, containing "STRUCTURE_3uso", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_3uso| PDB=3uso | SCENE= }}
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===Crystal structure of LeuT bound to L-selenomethionine in space group P21212 from lipid bicelles===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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*[[Leucine transporter|Leucine transporter]]
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The line below this paragraph, {{ABSTRACT_PUBMED_22245965}}, adds the Publication Abstract to the page
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== References ==
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(as it appears on PubMed at http://www.pubmed.gov), where 22245965 is the PubMed ID number.
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<references/>
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__TOC__
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{{ABSTRACT_PUBMED_22245965}}
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</StructureSection>
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==About this Structure==
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[[3uso]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3USO OCA].
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==Reference==
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<ref group="xtra">PMID:022245965</ref><references group="xtra"/>
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[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
[[Category: Elferich, J.]]
[[Category: Elferich, J.]]

Revision as of 06:34, 5 June 2014

Crystal structure of LeuT bound to L-selenomethionine in space group P21212 from lipid bicelles

3uso, resolution 4.50Å

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