4dzs

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[[Image:4dzs.png|left|200px]]
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==Crystal structure of yeast Puf4p RNA binding domain in complex with HO-4BE mutant RNA==
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<StructureSection load='4dzs' size='340' side='right' caption='[[4dzs]], [[Resolution|resolution]] 3.14&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4dzs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DZS FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bx2|3bx2]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PUF4, YGL014W, YGL023 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dzs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dzs RCSB], [http://www.ebi.ac.uk/pdbsum/4dzs PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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mRNA control hinges on the specificity and affinity of proteins for their RNA binding sites. Regulatory proteins must bind their own sites and reject even closely related noncognate sites. In the PUF [Pumilio and fem-3 binding factor (FBF)] family of RNA binding proteins, individual proteins discriminate differences in the length and sequence of binding sites, allowing each PUF to bind a distinct battery of mRNAs. Here, we show that despite these differences, the pattern of RNA interactions is conserved among PUF proteins: the two ends of the PUF protein make critical contacts with the two ends of the RNA sites. Despite this conserved "two-handed" pattern of recognition, the RNA sequence is flexible. Among the binding sites of yeast Puf4p, RNA sequence dictates the pattern in which RNA bases are flipped away from the binding surface of the protein. Small differences in RNA sequence allow new modes of control, recruiting Puf5p in addition to Puf4p to a single site. This embedded information adds a new layer of biological meaning to the connections between RNA targets and PUF proteins.
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Patterns and plasticity in RNA-protein interactions enable recruitment of multiple proteins through a single site.,Valley CT, Porter DF, Qiu C, Campbell ZT, Hall TM, Wickens M Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6054-9. Epub 2012 Mar 30. PMID:22467831<ref>PMID:22467831</ref>
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The line below this paragraph, containing "STRUCTURE_4dzs", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_4dzs| PDB=4dzs | SCENE= }}
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===Crystal structure of yeast Puf4p RNA binding domain in complex with HO-4BE mutant RNA===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22467831}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 22467831 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_22467831}}
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==About this Structure==
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[[4dzs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZS OCA].
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==Reference==
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<ref group="xtra">PMID:022467831</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Hall, T M.T]]
[[Category: Hall, T M.T]]

Revision as of 07:16, 5 June 2014

Crystal structure of yeast Puf4p RNA binding domain in complex with HO-4BE mutant RNA

4dzs, resolution 3.14Å

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