1b69
From Proteopedia
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| - | [[Image:1b69.gif|left|200px]] | + | [[Image:1b69.gif|left|200px]] |
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| - | '''THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX''' | + | {{Structure |
| + | |PDB= 1b69 |SIZE=350|CAPTION= <scene name='initialview01'>1b69</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1B69 is a [ | + | 1B69 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B69 OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structure of the Tn916 integrase-DNA complex., Wojciak JM, Connolly KM, Clubb RT, Nat Struct Biol. 1999 Apr;6(4):366-73. PMID:[http:// | + | NMR structure of the Tn916 integrase-DNA complex., Wojciak JM, Connolly KM, Clubb RT, Nat Struct Biol. 1999 Apr;6(4):366-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10201406 10201406] |
[[Category: Enterococcus faecalis]] | [[Category: Enterococcus faecalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transposition]] | [[Category: transposition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:06:51 2008'' |
Revision as of 08:06, 20 March 2008
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THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX
Overview
The integrase protein catalyzes the excision and integration of the Tn916 conjugative transposon, a promiscuous genetic element that spreads antibiotic resistance in pathogenic bacteria. The solution structure of the N-terminal domain of the Tn916 integrase protein bound to its DNA-binding site within the transposon arm has been determined. The structure reveals an interesting mode of DNA recognition, in which the face of a three-stranded antiparallel beta-sheet is positioned within the major groove. A comparison to the structure of the homing endonuclease I-Ppol-DNA complex suggests that the three-stranded sheet may represent a new DNA-binding motif whose residue composition and position within the major groove are varied to alter specificity. The structure also provides insights into the mechanism of conjugative transposition. The DNA in the complex is bent approximately 35 degrees and may, together with potential interactions between bound integrase proteins at directly repeated sites, significantly bend the arms of the transposon.
About this Structure
1B69 is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.
Reference
NMR structure of the Tn916 integrase-DNA complex., Wojciak JM, Connolly KM, Clubb RT, Nat Struct Biol. 1999 Apr;6(4):366-73. PMID:10201406
Page seeded by OCA on Thu Mar 20 10:06:51 2008
