1b8j
From Proteopedia
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| - | [[Image:1b8j.gif|left|200px]] | + | [[Image:1b8j.gif|left|200px]] |
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| - | '''ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE''' | + | {{Structure |
| + | |PDB= 1b8j |SIZE=350|CAPTION= <scene name='initialview01'>1b8j</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] | ||
| + | |GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1B8J is a [ | + | 1B8J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8J OCA]. |
==Reference== | ==Reference== | ||
| - | A model of the transition state in the alkaline phosphatase reaction., Holtz KM, Stec B, Kantrowitz ER, J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:[http:// | + | A model of the transition state in the alkaline phosphatase reaction., Holtz KM, Stec B, Kantrowitz ER, J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10085061 10085061] |
[[Category: Alkaline phosphatase]] | [[Category: Alkaline phosphatase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: vanadate]] | [[Category: vanadate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:07:44 2008'' |
Revision as of 08:07, 20 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | PHOA (Escherichia coli) | ||||||
| Activity: | Alkaline phosphatase, with EC number 3.1.3.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE
Overview
A high resolution crystal structure of Escherichia coli alkaline phosphatase in the presence of vanadate has been refined to 1.9 A resolution. The vanadate ion takes on a trigonal bipyramidal geometry and is covalently bound by the active site serine nucleophile. A coordinated water molecule occupies the axial position opposite the serine nucleophile, whereas the equatorial oxygen atoms of the vanadate ion are stabilized by interactions with both Arg-166 and the zinc metal ions of the active site. This structural complex supports the in-line displacement mechanism of phosphomonoester hydrolysis by alkaline phosphatase and provides a model for the proposed transition state in the enzyme-catalyzed reaction.
About this Structure
1B8J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A model of the transition state in the alkaline phosphatase reaction., Holtz KM, Stec B, Kantrowitz ER, J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:10085061
Page seeded by OCA on Thu Mar 20 10:07:44 2008
