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4dbl

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[[Image:4dbl.png|left|200px]]
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==Crystal structure of E159Q mutant of BtuCDF==
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<StructureSection load='4dbl' size='340' side='right' caption='[[4dbl]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4dbl]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DBL FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vitamin_B12-transporting_ATPase Vitamin B12-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.33 3.6.3.33] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dbl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dbl RCSB], [http://www.ebi.ac.uk/pdbsum/4dbl PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BtuCD is an ABC transporter catalyzing the uptake of vitamin B(12) across the Escherichia coli inner membrane. A previously reported X-ray structure of BtuCD in complex with the periplasmic vitamin B(12)-binding protein BtuF revealed asymmetry of the transmembrane BtuC subunits. The functional relevance of this asymmetry has remained uncertain. Here we report the X-ray structure of a catalytically impaired BtuCD mutant in complex with BtuF, where the BtuC subunits adopt a distinct asymmetric conformation. The structure suggests that BtuF does not discriminate between, or impose, asymmetric conformations of BtuCD. It also explains the conformational disorder observed in BtuCDF crystals.
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Asymmetric states of vitamin B12 transporter BtuCD are not discriminated by its cognate substrate binding protein BtuF.,Korkhov VM, Mireku SA, Hvorup RN, Locher KP FEBS Lett. 2012 Apr 5;586(7):972-6. Epub 2012 Mar 8. PMID:22569249<ref>PMID:22569249</ref>
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The line below this paragraph, containing "STRUCTURE_4dbl", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_4dbl| PDB=4dbl | SCENE= }}
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===Crystal structure of E159Q mutant of BtuCDF===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22569249}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 22569249 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_22569249}}
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==About this Structure==
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[[4dbl]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBL OCA].
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==Reference==
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<ref group="xtra">PMID:022569249</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Vitamin B12-transporting ATPase]]
[[Category: Vitamin B12-transporting ATPase]]

Revision as of 07:41, 5 June 2014

Crystal structure of E159Q mutant of BtuCDF

4dbl, resolution 3.49Å

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