1bbx
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1bbx.jpg|left|200px]] | + | [[Image:1bbx.jpg|left|200px]] |
| - | + | ||
| - | '''NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, NMR, 1 STRUCTURE''' | + | {{Structure |
| + | |PDB= 1bbx |SIZE=350|CAPTION= <scene name='initialview01'>1bbx</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, NMR, 1 STRUCTURE''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1BBX is a [ | + | 1BBX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BBX OCA]. |
==Reference== | ==Reference== | ||
| - | Architecture of nonspecific protein-DNA interactions in the Sso7d-DNA complex., Agback P, Baumann H, Knapp S, Ladenstein R, Hard T, Nat Struct Biol. 1998 Jul;5(7):579-84. PMID:[http:// | + | Architecture of nonspecific protein-DNA interactions in the Sso7d-DNA complex., Agback P, Baumann H, Knapp S, Ladenstein R, Hard T, Nat Struct Biol. 1998 Jul;5(7):579-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9665172 9665172] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
| Line 22: | Line 31: | ||
[[Category: protein-dna interaction]] | [[Category: protein-dna interaction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:08:55 2008'' |
Revision as of 08:08, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, NMR, 1 STRUCTURE
Overview
Many biochemical processes, including DNA packing, maintenance and control, rely on non-sequence specific protein-DNA interactions. Nonspecific DNA-binding proteins have evolved to tolerate a wide range of DNA sequences, yet bind with a respectable affinity. The nonspecific binding requirement is in contrast to that imposed on, for example, transcription factors and implies a different structural basis for the biomolecular recognition process. To address this issue, and the mechanism for archaeal DNA packing, we determined the structure of the Sso7d protein from Sulfolobus solfataricus in complex with DNA. Sso7d binds DNA by placing a triple-stranded beta-sheet across the DNA minor groove. The protein is anchored in this position by the insertion of hydrogen bond-donating side chains into the groove and additionally stabilized by electrostatic and non-polar interactions with the DNA backbone. This structure explains how strong binding can be achieved independent of DNA sequence. Sso7d binding also distorts the DNA conformation and introduces significant unwinding of the helix. This effect suggests a mechanism for DNA packing in Sulfolobus based on negative DNA supercoiling.
About this Structure
1BBX is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Architecture of nonspecific protein-DNA interactions in the Sso7d-DNA complex., Agback P, Baumann H, Knapp S, Ladenstein R, Hard T, Nat Struct Biol. 1998 Jul;5(7):579-84. PMID:9665172
Page seeded by OCA on Thu Mar 20 10:08:55 2008
