1bcu
From Proteopedia
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| - | [[Image:1bcu.jpg|left|200px]] | + | [[Image:1bcu.jpg|left|200px]] |
| - | + | ||
| - | '''ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN''' | + | {{Structure |
| + | |PDB= 1bcu |SIZE=350|CAPTION= <scene name='initialview01'>1bcu</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BCU is a [ | + | 1BCU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCU OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray and spectrophotometric studies of the binding of proflavin to the S1 specificity pocket of human alpha-thrombin., Conti E, Rivetti C, Wonacott A, Brick P, FEBS Lett. 1998 Mar 27;425(2):229-33. PMID:[http:// | + | X-ray and spectrophotometric studies of the binding of proflavin to the S1 specificity pocket of human alpha-thrombin., Conti E, Rivetti C, Wonacott A, Brick P, FEBS Lett. 1998 Mar 27;425(2):229-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9559654 9559654] |
[[Category: Hirudo medicinalis]] | [[Category: Hirudo medicinalis]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:09:17 2008'' |
Revision as of 08:09, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Thrombin, with EC number 3.4.21.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN
Contents |
Overview
Proflavin can be used to study the interactions of inhibitors and substrates with thrombin by monitoring the changes in the visible absorption spectrum that occur on dye displacement. We have used microspectrophotometric methods to investigate the binding of proflavin to crystals of an alpha-thrombin-hirugen complex and have determined the structure by X-ray crystallography. The proflavin molecule binds in the S1 pocket of the enzyme with one of the amino groups hydrogen bonded to the carboxylate of Asp-189 while the protonated ring nitrogen is hydrogen bonded to the carbonyl of Gly-219. This result indicates that the proflavin displacement assay can be used to specifically monitor the binding of inhibitors to the S1 pocket.
Disease
Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]
About this Structure
1BCU is a Protein complex structure of sequences from Hirudo medicinalis and Homo sapiens. Full crystallographic information is available from OCA.
Reference
X-ray and spectrophotometric studies of the binding of proflavin to the S1 specificity pocket of human alpha-thrombin., Conti E, Rivetti C, Wonacott A, Brick P, FEBS Lett. 1998 Mar 27;425(2):229-33. PMID:9559654
Page seeded by OCA on Thu Mar 20 10:09:17 2008
