4dzo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:4dzo.png|left|200px]]
+
==Structure of Human Mad1 C-terminal Domain Reveals Its Involvement in Kinetochore Targeting==
 +
<StructureSection load='4dzo' size='340' side='right' caption='[[4dzo]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4dzo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DZO FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MAD1, MAD1L1, TXBP181 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dzo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dzo RCSB], [http://www.ebi.ac.uk/pdbsum/4dzo PDBsum]</span></td></tr>
 +
<table>
 +
== Disease ==
 +
[[http://www.uniprot.org/uniprot/MD1L1_HUMAN MD1L1_HUMAN]] Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
 +
== Function ==
 +
[[http://www.uniprot.org/uniprot/MD1L1_HUMAN MD1L1_HUMAN]] Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.<ref>PMID:10049595</ref> <ref>PMID:12837246</ref> <ref>PMID:20133940</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The spindle checkpoint prevents aneuploidy by delaying anaphase onset until all sister chromatids achieve proper microtubule attachment. The kinetochore-bound checkpoint protein complex Mad1-Mad2 promotes the conformational activation of Mad2 and serves as a catalytic engine of checkpoint signaling. How Mad1 is targeted to kinetochores is not understood. Here, we report the crystal structure of the conserved C-terminal domain (CTD) of human Mad1. Mad1 CTD forms a homodimer and, unexpectedly, has a fold similar to those of the kinetochore-binding domains of Spc25 and Csm1. Nonoverlapping Mad1 fragments retain detectable kinetochore targeting. Deletion of the CTD diminishes, does not abolish, Mad1 kinetochore localization. Mutagenesis studies further map the functional interface of Mad1 CTD in kinetochore targeting and implicate Bub1 as its receptor. Our results indicate that CTD is a part of an extensive kinetochore-binding interface of Mad1, and rationalize graded kinetochore targeting of Mad1 during checkpoint signaling.
-
<!--
+
Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting.,Kim S, Sun H, Tomchick DR, Yu H, Luo X Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6549-54. Epub 2012 Apr 9. PMID:22493223<ref>PMID:22493223</ref>
-
The line below this paragraph, containing "STRUCTURE_4dzo", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_4dzo| PDB=4dzo | SCENE= }}
+
-
===Structure of Human Mad1 C-terminal Domain Reveals Its Involvement in Kinetochore Targeting===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
 
+
== References ==
-
<!--
+
<references/>
-
The line below this paragraph, {{ABSTRACT_PUBMED_22493223}}, adds the Publication Abstract to the page
+
__TOC__
-
(as it appears on PubMed at http://www.pubmed.gov), where 22493223 is the PubMed ID number.
+
</StructureSection>
-
-->
+
-
{{ABSTRACT_PUBMED_22493223}}
+
-
 
+
-
==About this Structure==
+
-
[[4dzo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZO OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:022493223</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Luo, X.]]
[[Category: Luo, X.]]

Revision as of 08:08, 5 June 2014

Structure of Human Mad1 C-terminal Domain Reveals Its Involvement in Kinetochore Targeting

4dzo, resolution 1.76Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4dzo"
Views
Personal tools
Navigation
Toolbox