4pga

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==GLUTAMINASE-ASPARAGINASE FROM PSEUDOMONAS 7A==
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[[Image:4pga.png|left|200px]]
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<StructureSection load='4pga' size='340' side='right' caption='[[4pga]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4pga]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._7a Pseudomonas sp. 7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PGA FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamin-(asparagin-)ase Glutamin-(asparagin-)ase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.38 3.5.1.38] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pga OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pga RCSB], [http://www.ebi.ac.uk/pdbsum/4pga PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pg/4pga_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pseudomonas 7A glutaminase-asparaginase (PGA) catalyzes the hydrolysis of D- and L-isomers of glutamine and asparagine. X-ray quality type-1 crystals of PGA have been obtained from 2.0 M ammonium sulfate. The space group is C222(1) with unit-cell dimensions a = 78.62, b = 135.80, and c = 137.88 A. The tetrameric molecule is located on a crystallographic 2-fold axis, and two subunits form the asymmetric portion of the unit cell. The structure was solved by the molecular replacement method and refined at 1.7 A resolution to an R = 19.9% with a good geometry of the model, G = 0.05. The resultant electron density maps enabled us to resolve individual constituent atoms of most residues and introduce minor revisions to the amino acid sequence. The catalytic loop, Thr20-Gly40, is in the closed conformation with excellent electron density in both subunits. A sulfate ion and an ammonium ion are bound in the substrate binding site and interect with the loop. This interaction appears to be responsible for the observed closed conformation. New arguments supporting Thr20 as the catalytic nucleophile in the asparaginase activity are proposed.
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Ion binding induces closed conformation in Pseudomonas 7A glutaminase-asparaginase (PGA): crystal structure of the PGA-SO4(2-)-NH4+ complex at 1.7 A resolution.,Jakob CG, Lewinski K, LaCount MW, Roberts J, Lebioda L Biochemistry. 1997 Jan 28;36(4):923-31. PMID:9020792<ref>PMID:9020792</ref>
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The line below this paragraph, containing "STRUCTURE_4pga", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_4pga| PDB=4pga | SCENE= }}
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===GLUTAMINASE-ASPARAGINASE FROM PSEUDOMONAS 7A===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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*[[Glutaminase|Glutaminase]]
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The line below this paragraph, {{ABSTRACT_PUBMED_9020792}}, adds the Publication Abstract to the page
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== References ==
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(as it appears on PubMed at http://www.pubmed.gov), where 9020792 is the PubMed ID number.
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<references/>
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__TOC__
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{{ABSTRACT_PUBMED_9020792}}
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</StructureSection>
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==About this Structure==
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4PGA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_sp._7a Pseudomonas sp. 7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PGA OCA].
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==Reference==
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<ref group="xtra">PMID:9020792</ref><references group="xtra"/>
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[[Category: Pseudomonas sp. 7a]]
[[Category: Pseudomonas sp. 7a]]
[[Category: Jakob, C G.]]
[[Category: Jakob, C G.]]
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[[Category: Roberts, J.]]
[[Category: Roberts, J.]]
[[Category: Bacterial amidohydrolase]]
[[Category: Bacterial amidohydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:23:31 2009''
 

Revision as of 08:11, 5 June 2014

GLUTAMINASE-ASPARAGINASE FROM PSEUDOMONAS 7A

4pga, resolution 1.70Å

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