4e5k

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[[Image:4e5k.png|left|200px]]
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==Thermostable phosphite dehydrogenase in complex with NAD and sulfite==
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<StructureSection load='4e5k' size='340' side='right' caption='[[4e5k]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4e5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E5K FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e5m|4e5m]], [[4e5n|4e5n]], [[4e5p|4e5p]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e5k RCSB], [http://www.ebi.ac.uk/pdbsum/4e5k PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzyme phosphite dehydrogenase (PTDH) catalyzes the NAD(+)-dependent conversion of phosphite to phosphate and represents the first biological catalyst that has been shown to conduct the enzymatic oxidation of phosphorus. Despite investigation for more than a decade into both the mechanism of its unusual reaction and its utility in cofactor regeneration, there has been a lack of any structural data for PTDH. Here we present the cocrystal structure of an engineered thermostable variant of PTDH bound to NAD(+) (1.7 A resolution), as well as four other cocrystal structures of thermostable PTDH and its variants with different ligands (all between 1.85 and 2.3 A resolution). These structures provide a molecular framework for understanding prior mutational analysis and point to additional residues, located in the active site, that may contribute to the enzymatic activity of this highly unusual catalyst.
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Crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration.,Zou Y, Zhang H, Brunzelle JS, Johannes TW, Woodyer R, Hung JE, Nair N, van der Donk WA, Zhao H, Nair SK Biochemistry. 2012 May 29;51(21):4263-70. Epub 2012 May 17. PMID:22564171<ref>PMID:22564171</ref>
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The line below this paragraph, containing "STRUCTURE_4e5k", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_4e5k| PDB=4e5k | SCENE= }}
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===Thermostable phosphite dehydrogenase in complex with NAD and sulfite===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22564171}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 22564171 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_22564171}}
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==About this Structure==
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[[4e5k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E5K OCA].
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==Reference==
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<ref group="xtra">PMID:022564171</ref><references group="xtra"/>
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[[Category: Pseudomonas stutzeri]]
[[Category: Pseudomonas stutzeri]]
[[Category: Nair, S K.]]
[[Category: Nair, S K.]]

Revision as of 08:18, 5 June 2014

Thermostable phosphite dehydrogenase in complex with NAD and sulfite

4e5k, resolution 1.95Å

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