4f3e
From Proteopedia
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| - | [[ | + | ==Crystal Structure of Thermus thermophilus HB8 CasA== |
| + | <StructureSection load='4f3e' size='340' side='right' caption='[[4f3e]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4f3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F3E FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHB188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f3e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f3e RCSB], [http://www.ebi.ac.uk/pdbsum/4f3e PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Prokaryotes make use of small RNAs encoded by CRISPR (clustered regularly interspaced short palindromic repeat) loci to provide immunity against bacteriophage or plasmid invasion. In Escherichia coli, the CRISPR-associated Complex for Antiviral Defense (Cascade) utilizes these RNAs to target foreign DNA for destruction. CasA, the largest subunit of Cascade, is essential for its function. Here we report the crystal structure of Thermus thermophilus CasA. The structure is composed of two domains that are arranged in a chair-like conformation with a novel fold forming the larger N-terminal domain. Docking of the crystal structure into cryo-electron microscopy maps reveals two loops in CasA likely have important functions in DNA target binding. Finally, DNA binding experiments show CasA is essential for binding of Cascade to DNA target. | ||
| - | + | Crystal structure of the largest subunit of a bacterial RNA-guided immune complex and its role in DNA target binding.,Mulepati S, Orr A, Bailey S J Biol Chem. 2012 May 23. PMID:22621933<ref>PMID:22621933</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
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| - | == | + | |
| - | < | + | |
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Bailey, S.]] | [[Category: Bailey, S.]] | ||
Revision as of 08:26, 5 June 2014
Crystal Structure of Thermus thermophilus HB8 CasA
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