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1bfn

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[[Image:1bfn.gif|left|200px]]<br /><applet load="1bfn" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1bfn.gif|left|200px]]
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caption="1bfn, resolution 2.07&Aring;" />
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'''BETA-AMYLASE/BETA-CYCLODEXTRIN COMPLEX'''<br />
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{{Structure
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|PDB= 1bfn |SIZE=350|CAPTION= <scene name='initialview01'>1bfn</scene>, resolution 2.07&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2]
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|GENE=
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}}
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'''BETA-AMYLASE/BETA-CYCLODEXTRIN COMPLEX'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1BFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BFN OCA].
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1BFN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BFN OCA].
==Reference==
==Reference==
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Crystal structure of recombinant soybean beta-amylase complexed with beta-cyclodextrin., Adachi M, Mikami B, Katsube T, Utsumi S, J Biol Chem. 1998 Jul 31;273(31):19859-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9677422 9677422]
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Crystal structure of recombinant soybean beta-amylase complexed with beta-cyclodextrin., Adachi M, Mikami B, Katsube T, Utsumi S, J Biol Chem. 1998 Jul 31;273(31):19859-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9677422 9677422]
[[Category: Beta-amylase]]
[[Category: Beta-amylase]]
[[Category: Glycine max]]
[[Category: Glycine max]]
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[[Category: recombinant]]
[[Category: recombinant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:10:22 2008''

Revision as of 08:10, 20 March 2008

Image:1bfn.gif


PDB ID 1bfn

Drag the structure with the mouse to rotate
, resolution 2.07Å
Ligands:
Activity: Beta-amylase, with EC number 3.2.1.2
Coordinates: save as pdb, mmCIF, xml



BETA-AMYLASE/BETA-CYCLODEXTRIN COMPLEX


Overview

In order to study the interaction of soybean beta-amylase with substrate, we solved the crystal structure of beta-cyclodextrin-enzyme complex and compared it with that of alpha-cyclodextrin-enzyme complex. The enzyme was expressed in Escherichia coli at a high level as a soluble and catalytically active protein. The purified recombinant enzyme had properties nearly identical to those of native soybean beta-amylase and formed the same crystals as the native enzyme. The crystal structure of recombinant enzyme complexed with beta-cyclodextrin was refined at 2. 07-A resolution with a final crystallographic R value of 15.8% (Rfree = 21.1%). The root mean square deviation in the position of C-alpha atoms between this recombinant enzyme and the native enzyme was 0.22 A. These results indicate that the expression system established here is suitable for studying structure-function relationships of beta-amylase. The conformation of the bound beta-cyclodextrin takes an ellipsoid shape in contrast to the circular shape of the bound alpha-cyclodextrin. The cyclodextrins shared mainly two glucose binding sites, 3 and 4. The glucose residue 4 was slightly shifted from the maltose binding site. This suggests that the binding site of the cyclodextrins is important for its holding of a cleaved substrate, which enables the multiple attack mechanism of beta-amylase.

About this Structure

1BFN is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.

Reference

Crystal structure of recombinant soybean beta-amylase complexed with beta-cyclodextrin., Adachi M, Mikami B, Katsube T, Utsumi S, J Biol Chem. 1998 Jul 31;273(31):19859-65. PMID:9677422

Page seeded by OCA on Thu Mar 20 10:10:22 2008

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