9mht

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{{Seed}}
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==CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX==
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[[Image:9mht.png|left|200px]]
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<StructureSection load='9mht' size='340' side='right' caption='[[9mht]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[9mht]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9MHT FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9mht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9mht OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9mht RCSB], [http://www.ebi.ac.uk/pdbsum/9mht PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/9mht_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Three structures have been determined for complexes between HhaI methyltransferase (M.HhaI) and oligonucleotides containing a G:A, G:U or G:AP (AP = abasic or apurinic/apyrimidinic) mismatch at the target base pair. The mismatched adenine, uracil and abasic site are all flipped out of the DNA helix and located in the enzyme's active-site pocket, adopting the same conformation as in the flipped-out normal substrate. These results, particularly the flipped-out abasic deoxyribose sugar, provide insight into the mechanism of base flipping. If the process involves the protein pushing the base out of the helix, then the push must take place not on the base, but rather on the sugar-phosphate backbone. Thus rotation of the DNA backbone is probably the key to base flipping.
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<!--
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Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base.,O'Gara M, Horton JR, Roberts RJ, Cheng X Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:9783745<ref>PMID:9783745</ref>
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The line below this paragraph, containing "STRUCTURE_9mht", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_9mht| PDB=9mht | SCENE= }}
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===CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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<!--
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*[[HhaI DNA methyltransferase|HhaI DNA methyltransferase]]
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The line below this paragraph, {{ABSTRACT_PUBMED_9783745}}, adds the Publication Abstract to the page
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== References ==
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(as it appears on PubMed at http://www.pubmed.gov), where 9783745 is the PubMed ID number.
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<references/>
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-->
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__TOC__
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{{ABSTRACT_PUBMED_9783745}}
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</StructureSection>
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==About this Structure==
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9MHT is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MHT OCA].
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==Reference==
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<ref group="xtra">PMID:9783745</ref><references group="xtra"/>
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[[Category: Deleted entry]]
[[Category: Deleted entry]]
[[Category: Haemophilus haemolyticus]]
[[Category: Haemophilus haemolyticus]]
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[[Category: Restriction system]]
[[Category: Restriction system]]
[[Category: Transferase]]
[[Category: Transferase]]
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[[Category: Transferase-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:09:38 2009''
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Revision as of 09:17, 5 June 2014

CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX

9mht, resolution 2.39Å

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OCA

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