1m9g

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[[Image:1m9g.png|left|200px]]
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==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==
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<StructureSection load='1m9g' size='340' side='right' caption='[[1m9g]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mechanism by which sweet proteins elicit a response on the T1R2-T1R3 sweet taste receptor is still mostly unknown but has been so far related to the presence of "sweet fingers" on the protein surface able to interact with the same mechanism as that of low molecular mass sweeteners. In the search for the identification of sweet fingers, we have solved the solution structure of G16A MNEI, a structural mutant that shows a reduction of one order of magnitude in sweetness with respect to its parent protein, MNEI, a single-chain monellin. Comparison of the structures of wild-type monellin and its G16A mutant shows that the mutation does not affect the structure of potential glucophores but produces a distortion of the surface owing to the partial relative displacement of elements of secondary structure. These results show conclusively that sweet proteins do not possess a sweet finger and strongly support the hypothesis that the mechanism of interaction of sweet-tasting proteins with the recently identified T1R2-T1R3 GPC receptor is different from that of low molecular mass sweeteners.
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{{STRUCTURE_1m9g| PDB=1m9g | SCENE= }}
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The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI.,Spadaccini R, Trabucco F, Saviano G, Picone D, Crescenzi O, Tancredi T, Temussi PA J Mol Biol. 2003 May 2;328(3):683-92. PMID:12706725<ref>PMID:12706725</ref>
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===Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_12706725}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA].
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</StructureSection>
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[[Category: Diocu]]
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==Reference==
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<ref group="xtra">PMID:012706725</ref><references group="xtra"/>
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[[Category: Dioscoreophyllum cumminsii]]
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[[Category: Crescenzi, O.]]
[[Category: Crescenzi, O.]]
[[Category: Picone, D.]]
[[Category: Picone, D.]]

Revision as of 05:23, 8 June 2014

Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI

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