1kcn

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{{Seed}}
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==Structure of e109 Zeta Peptide, an Antagonist of the High-Affinity IgE Receptor==
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[[Image:1kcn.png|left|200px]]
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<StructureSection load='1kcn' size='340' side='right' caption='[[1kcn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kcn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KCN FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kco|1kco]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kcn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kcn RCSB], [http://www.ebi.ac.uk/pdbsum/1kcn PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Recently we described a family of peptides, unrelated in sequence to IgE, that form stable beta-hairpins in solution and inhibit IgE activity in the microM range [Nakamura, G. R., Starovasnik, M. A., Reynolds, M. E. &amp; Lowman, H. B. (2001) Biochemistry 40, 9828-9835]. Using an expanded set of peptide-phage libraries, we found a simpler motif, X(2)CPX(2)CYX, for binding to the high-affinity IgE receptor. In solution, one of these peptides spontaneously formed a covalent antiparallel dimer. We subsequently linked these monomers in a single-chain construct on phage and optimized receptor binding. Ultimately, peptides with 30 nM affinity were produced. NMR studies showed that the peptide adopts a stable fold consisting of two "zeta" (zeta)-shaped moieties. Structure-activity analyses reveal a single binding site created by the zeta-dimer, with two tyrosine residues important for structural stability and two proline residues important for Fc epsilon RI binding. The peptides inhibit histamine release from cultured cells and are extremely stable in biological fluids. The zeta peptides appear to act as competitive IgE inhibitors and suggest possibilities for design of novel IgE antagonists.
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Stable "zeta" peptides that act as potent antagonists of the high-affinity IgE receptor.,Nakamura GR, Reynolds ME, Chen YM, Starovasnik MA, Lowman HB Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1303-8. PMID:11830661<ref>PMID:11830661</ref>
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The line below this paragraph, containing "STRUCTURE_1kcn", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_1kcn| PDB=1kcn | SCENE= }}
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===Structure of e109 Zeta Peptide, an Antagonist of the High-Affinity IgE Receptor===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_11830661}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 11830661 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_11830661}}
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==About this Structure==
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCN OCA].
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==Reference==
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Stable "zeta" peptides that act as potent antagonists of the high-affinity IgE receptor., Nakamura GR, Reynolds ME, Chen YM, Starovasnik MA, Lowman HB, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1303-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11830661 11830661]
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[[Category: Chen, Y M.]]
[[Category: Chen, Y M.]]
[[Category: Lowman, H B.]]
[[Category: Lowman, H B.]]
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[[Category: Disulfide-bonded]]
[[Category: Disulfide-bonded]]
[[Category: Helical]]
[[Category: Helical]]
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[[Category: Protein binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:08:06 2008''
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Revision as of 05:31, 8 June 2014

Structure of e109 Zeta Peptide, an Antagonist of the High-Affinity IgE Receptor

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