1ybk
From Proteopedia
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| - | [[ | + | ==RHCC cocrystallized with CAPB== |
| + | <StructureSection load='1ybk' size='340' side='right' caption='[[1ybk]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1ybk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YBK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YBK FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEQ:N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM+INNER+SALT'>BEQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fe6|1fe6]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ybk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ybk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ybk RCSB], [http://www.ebi.ac.uk/pdbsum/1ybk PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A. | ||
| - | + | Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB).,Ozbek S, Muller JF, Figgemeier E, Stetefeld J Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:15805603<ref>PMID:15805603</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Staphylothermus marinus]] | [[Category: Staphylothermus marinus]] | ||
[[Category: Stetefeld, J.]] | [[Category: Stetefeld, J.]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Right hand coiled coil]] | [[Category: Right hand coiled coil]] | ||
Revision as of 07:08, 9 June 2014
RHCC cocrystallized with CAPB
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