413d

Publication Abstract from PubMed

Here we demonstrate the presence of the A'-RNA conformation using the single crystal structure of a tridecamer: r(UGAGCUUCGGCUC). The average A'-RNA conformation deduced from X-ray fiber diffraction data had only been available previously, but now the presence of the A'-RNA conformation has been found in a single crystal structure for the first time. Statistical analysis showed that the A'-RNA conformation is distinguishable from the A-RNA conformation in a plot of the major groove width against the base pair inclination angle. The major groove of the A'-RNA conformation is wide enough to accommodate a protein or peptide while that of the A-RNA conformation is too narrow to do so. The presence of the A'-RNA conformation is significant for protein-RNA interaction.

A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC).,Tanaka Y, Fujii S, Hiroaki H, Sakata T, Tanaka T, Uesugi S, Tomita K, Kyogoku Y Nucleic Acids Res. 1999 Feb 15;27(4):949-55. PMID:9927725^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.