1c7y
From Proteopedia
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| - | [[Image:1c7y.gif|left|200px]] | + | [[Image:1c7y.gif|left|200px]] |
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| - | '''E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX''' | + | {{Structure |
| + | |PDB= 1c7y |SIZE=350|CAPTION= <scene name='initialview01'>1c7y</scene>, resolution 3.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1C7Y is a [ | + | 1C7Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7Y OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer., Ariyoshi M, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8257-62. PMID:[http:// | + | Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer., Ariyoshi M, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8257-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10890893 10890893] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:21:04 2008'' |
Revision as of 08:21, 20 March 2008
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| , resolution 3.10Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX
Overview
In the major pathway of homologous DNA recombination in prokaryotic cells, the Holliday junction intermediate is processed through its association with RuvA, RuvB, and RuvC proteins. Specific binding of the RuvA tetramer to the Holliday junction is required for the RuvB motor protein to be loaded onto the junction DNA, and the RuvAB complex drives the ATP-dependent branch migration. We solved the crystal structure of the Holliday junction bound to a single Escherichia coli RuvA tetramer at 3.1-A resolution. In this complex, one side of DNA is accessible for cleavage by RuvC resolvase at the junction center. The refined junction DNA structure revealed an open concave architecture with a four-fold symmetry. Each arm, with B-form DNA, in the Holliday junction is predominantly recognized in the minor groove through hydrogen bonds with two repeated helix-hairpin-helix motifs of each RuvA subunit. The local conformation near the crossover point, where two base pairs are disrupted, suggests a possible scheme for successive base pair rearrangements, which may account for smooth Holliday junction movement without segmental unwinding.
About this Structure
1C7Y is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer., Ariyoshi M, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8257-62. PMID:10890893
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