1ca5

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[[Image:1ca5.gif|left|200px]]<br /><applet load="1ca5" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ca5.gif|left|200px]]
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caption="1ca5, resolution 2.2&Aring;" />
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'''INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA'''<br />
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{{Structure
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|PDB= 1ca5 |SIZE=350|CAPTION= <scene name='initialview01'>1ca5</scene>, resolution 2.2&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1CA5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA].
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1CA5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA].
==Reference==
==Reference==
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Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11031116 11031116]
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Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11031116 11031116]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Sulfolobus acidocaldarius]]
[[Category: Sulfolobus acidocaldarius]]
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[[Category: sso7d]]
[[Category: sso7d]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:04:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:21:50 2008''

Revision as of 08:21, 20 March 2008

Image:1ca5.gif


PDB ID 1ca5

Drag the structure with the mouse to rotate
, resolution 2.2Å
Coordinates: save as pdb, mmCIF, xml



INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA


Overview

Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.

About this Structure

1CA5 is a Single protein structure of sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA.

Reference

Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116

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