3nte

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{{STRUCTURE_3nte| PDB=3nte | SCENE= }}
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==Crystal Structure of the Wild-type Full-Length HIV-1 Capsid Protein==
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===Crystal Structure of the Wild-type Full-Length HIV-1 Capsid Protein===
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<StructureSection load='3nte' size='340' side='right' caption='[[3nte]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nte]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NTE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NTE FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=I3M:TRI-IODODE+ANION'>I3M</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CA, gag ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nte OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nte RCSB], [http://www.ebi.ac.uk/pdbsum/3nte PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The capsid (CA) protein plays crucial roles in HIV infection and replication, essential to viral maturation. The absence of high-resolution structural data on unassembled CA hinders the development of antivirals effective in inhibiting assembly. Unlike enzymes that have targetable, functional substrate-binding sites, the CA does not have a known site that affects catalytic or other innate activity, which can be more readily targeted in drug development efforts. We report the crystal structure of the HIV-1 CA, revealing the domain organization in the context of the wild-type full-length (FL) unassembled CA. The FL CA adopts an antiparallel dimer configuration, exhibiting a domain organization sterically incompatible with capsid assembly. A small compound, generated in situ during crystallization, is bound tightly at a hinge site ("H site"), indicating that binding at this interdomain region stabilizes the ADP conformation. Electron microscopy studies on nascent crystals reveal both dimeric and hexameric lattices coexisting within a single condition, in agreement with the interconvertibility of oligomeric forms and supporting the feasibility of promoting assembly-incompetent dimeric states. Solution characterization in the presence of the H-site ligand shows predominantly unassembled dimeric CA, even under conditions that promote assembly. Our structure elucidation of the HIV-1 FL CA and characterization of a potential allosteric binding site provides three-dimensional views of an assembly-defective conformation, a state targeted in, and thus directly relevant to, inhibitor development. Based on our findings, we propose an unprecedented means of preventing CA assembly, by "conformationally trapping" CA in assembly-incompetent conformational states induced by H-site binding.
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==Function==
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Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding.,Du S, Betts L, Yang R, Shi H, Concel J, Ahn J, Aiken C, Zhang P, Yeh JI J Mol Biol. 2011 Feb 25;406(3):371-86. doi: 10.1016/j.jmb.2010.11.027. Epub 2010 , Dec 10. PMID:21146540<ref>PMID:21146540</ref>
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[[http://www.uniprot.org/uniprot/Q77YG1_9HIV1 Q77YG1_9HIV1]] Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).[SAAS:SAAS012344_004_011858]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3nte]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NTE OCA].
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</div>
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[[Category: Human immunodeficiency virus 1]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: 9hiv1]]
[[Category: Betts, L.]]
[[Category: Betts, L.]]
[[Category: Yeh, J I.]]
[[Category: Yeh, J I.]]
[[Category: Viral protein]]
[[Category: Viral protein]]
[[Category: Wild type viral protein]]
[[Category: Wild type viral protein]]

Revision as of 06:31, 2 July 2014

Crystal Structure of the Wild-type Full-Length HIV-1 Capsid Protein

3nte, resolution 1.95Å

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