4f3m

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[[Image:4f3m.png|left|200px]]
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==Crystal structure of CRISPR-associated protein==
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<StructureSection load='4f3m' size='340' side='right' caption='[[4f3m]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4f3m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_halodurans Bacillus halodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F3M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F3M FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BH0337 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86665 Bacillus halodurans])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f3m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f3m RCSB], [http://www.ebi.ac.uk/pdbsum/4f3m PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Clustered regularly interspaced short palindromic repeats (CRISPRs), together with an operon of CRISPR-associated (Cas) proteins, form an RNA-based prokaryotic immune system against exogenous genetic elements. Cas5 family proteins are found in several type I CRISPR-Cas systems. Here, we report the molecular function of subtype I-C/Dvulg Cas5d from Bacillus halodurans. We show that Cas5d cleaves pre-crRNA into unit length by recognizing both the hairpin structure and the 3' single stranded sequence in the CRISPR repeat region. Cas5d structure reveals a ferredoxin domain-based architecture and a catalytic triad formed by Y46, K116, and H117 residues. We further show that after pre-crRNA processing, Cas5d assembles with crRNA, Csd1, and Csd2 proteins to form a multi-sub-unit interference complex similar to Escherichia coli Cascade (CRISPR-associated complex for antiviral defense) in architecture. Our results suggest that formation of a crRNA-presenting Cascade-like complex is likely a common theme among type I CRISPR subtypes.
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{{STRUCTURE_4f3m| PDB=4f3m | SCENE= }}
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Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System.,Nam KH, Haitjema C, Liu X, Ding F, Wang H, Delisa MP, Ke A Structure. 2012 Jul 24. PMID:22841292<ref>PMID:22841292</ref>
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===Crystal structure of CRISPR-associated protein===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22841292}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[4f3m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_halodurans Bacillus halodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F3M OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:022841292</ref><references group="xtra"/>
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[[Category: Bacillus halodurans]]
[[Category: Bacillus halodurans]]
[[Category: Ke, A.]]
[[Category: Ke, A.]]

Revision as of 09:10, 2 July 2014

Crystal structure of CRISPR-associated protein

4f3m, resolution 1.71Å

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