1cq4

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[[Image:1cq4.gif|left|200px]]<br /><applet load="1cq4" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1cq4.gif|left|200px]]
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caption="1cq4, resolution 1.80&Aring;" />
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'''CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59'''<br />
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{{Structure
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|PDB= 1cq4 |SIZE=350|CAPTION= <scene name='initialview01'>1cq4</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1CQ4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA].
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1CQ4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA].
==Reference==
==Reference==
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Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat., Chen YW, Stott K, Perutz MF, Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9990011 9990011]
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Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat., Chen YW, Stott K, Perutz MF, Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9990011 9990011]
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: subtilisin- chymotrypsin inhibitor-2]]
[[Category: subtilisin- chymotrypsin inhibitor-2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:08:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:27:28 2008''

Revision as of 08:27, 20 March 2008

Image:1cq4.gif


PDB ID 1cq4

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59


Overview

We have constructed mutants of chymotrypsin inhibitor 2 with short glutamine repeats inserted into its inhibitory loop. These mutants oligomerize when expressed in Escherichia coli. The dimer of a mutant with four glutamines now has been crystallized, and its structure has been solved by molecular replacement by using the wild-type monomer as a search model. The structure of each half of the dimer is found to be the same as that of the wild-type monomer, except around the glutamine insertion. It was proposed that the components of the oligomers are held together by hydrogen bonds between the main-chain and side-chain amides of the glutamine repeats. Instead, they appear to form by swapping domains on folding in E. coli, and the glutamine repeats connecting the components of the dimers are disordered.

About this Structure

1CQ4 is a Protein complex structure of sequences from Hordeum vulgare. Full crystallographic information is available from OCA.

Reference

Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat., Chen YW, Stott K, Perutz MF, Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:9990011

Page seeded by OCA on Thu Mar 20 10:27:28 2008

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