4e2o
From Proteopedia
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| - | + | ==Crystal structure of alpha-amylase from Geobacillus thermoleovorans, GTA, complexed with acarbose== | |
| - | === | + | <StructureSection load='4e2o' size='340' side='right' caption='[[4e2o]], [[Resolution|resolution]] 2.10Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4e2o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_thermoleovorans_ccb_us3_uf5 Geobacillus thermoleovorans ccb_us3_uf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2O FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACI:6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL'>ACI</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=G6D:6-DEOXY-ALPHA-D-GLUCOSE'>G6D</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTCCBUS3UF5_7870 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111068 Geobacillus thermoleovorans CCB_US3_UF5])</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2o RCSB], [http://www.ebi.ac.uk/pdbsum/4e2o PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A truncated form of an alpha-amylase, GTA, from thermophilic Geobacillus thermoleovorans CCB_US3_UF5 was biochemically and structurally characterized. The recombinant GTA, which lacked both the N- and C-terminal transmembrane regions, functioned optimally at 70 degrees C and pH 6.0. While enzyme activity was not enhanced by the addition of CaCl2, GTA's thermostability was significantly improved in the presence of CaCl2. The structure, in complex with an acarbose-derived pseudo-hexasaccharide, consists of the typical three domains and binds one Ca(2+) ion. This Ca(2+) ion was strongly bound and not chelated by EDTA. A predicted second Ca(2+)-binding site, however, was disordered. With limited subsites, two novel substrate-binding residues, Y147 and Y182, may help increase substrate affinity. No distinct starch-binding domain is present, although two regions rich in aromatic residues have been observed. GTA, with a smaller domain B and several shorter loops compared to other alpha-amylases, has one of the most compact alpha-amylase folds that may contribute greatly to its tight Ca(2+) binding and thermostability. | ||
| - | + | Crystal structure of a compact alpha-amylase from Geobacillus thermoleovorans.,Mok SC, Teh AH, Saito JA, Najimudin N, Alam M Enzyme Microb Technol. 2013 Jun 10;53(1):46-54. doi:, 10.1016/j.enzmictec.2013.03.009. Epub 2013 Mar 28. PMID:23683704<ref>PMID:23683704</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Amylase|Amylase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Alpha-amylase]] | [[Category: Alpha-amylase]] | ||
[[Category: Geobacillus thermoleovorans ccb_us3_uf5]] | [[Category: Geobacillus thermoleovorans ccb_us3_uf5]] | ||
| + | [[Category: Alam, M.]] | ||
[[Category: Mok, S C.]] | [[Category: Mok, S C.]] | ||
| + | [[Category: Najimudin, N.]] | ||
| + | [[Category: Saito, J A.]] | ||
[[Category: Teh, A H.]] | [[Category: Teh, A H.]] | ||
[[Category: Calcium binding]] | [[Category: Calcium binding]] | ||
[[Category: Hydrolase-hydrolase inhibitor complex]] | [[Category: Hydrolase-hydrolase inhibitor complex]] | ||
[[Category: Tim barrel]] | [[Category: Tim barrel]] | ||
Revision as of 06:42, 23 July 2014
Crystal structure of alpha-amylase from Geobacillus thermoleovorans, GTA, complexed with acarbose
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