2ch5

From Proteopedia

Revision as of 15:03, 30 October 2007

CRYSTAL STRUCTURE OF HUMAN N-ACETYLGLUCOSAMINE KINASE IN COMPLEX WITH N-ACETYLGLUCOSAMINE

Overview

N-Acetylglucosamine (GlcNAc), a major component of complex carbohydrates, is synthesized de novo or salvaged from lysosomally degraded, glycoconjugates and from nutritional sources. The salvage pathway requires, that GlcNAc kinase converts GlcNAc to GlcNAc-6-phosphate, a component, utilized in UDP-GlcNAc biosynthesis or energy metabolism. GlcNAc kinase, belongs to the sugar kinase/Hsp70/actin superfamily that catalyze, phosphoryl transfer from ATP to their respective substrates, and in most, cases catalysis is associated with a large conformational change in which, the N-terminal small and C-terminal large domains enclose the substrates., Here we report two crystal structures of homodimeric human GlcNAc kinase, one in complex with GlcNAc and the other in complex with ADP and glucose., The ... [(full description)]

About this Structure

2CH5 is a [Single protein] structure of sequence from [Homo sapiens] with NAG, NDG and GOL as [ligands]. Active as [N-acetylglucosamine kinase], with EC number [2.7.1.59]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structures of human N-Acetylglucosamine kinase in two complexes with N-Acetylglucosamine and with ADP/glucose: insights into substrate specificity and regulation., Weihofen WA, Berger M, Chen H, Saenger W, Hinderlich S, J Mol Biol. 2006 Dec 1;364(3):388-99. Epub 2006 Sep 3. PMID:17010375

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