1cx0
From Proteopedia
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| - | [[Image:1cx0.gif|left|200px]] | + | [[Image:1cx0.gif|left|200px]] |
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| - | '''HEPATITIS DELTA VIRUS RIBOZYME''' | + | {{Structure |
| + | |PDB= 1cx0 |SIZE=350|CAPTION= <scene name='initialview01'>1cx0</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''HEPATITIS DELTA VIRUS RIBOZYME''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1CX0 is a [ | + | 1CX0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX0 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:[http:// | + | Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9783582 9783582] |
[[Category: Hepatitis delta virus]] | [[Category: Hepatitis delta virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nested double pseudoknot rna structure]] | [[Category: nested double pseudoknot rna structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:30:01 2008'' |
Revision as of 08:30, 20 March 2008
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| , resolution 2.30Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
HEPATITIS DELTA VIRUS RIBOZYME
Overview
The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
About this Structure
1CX0 is a Single protein structure of sequence from Hepatitis delta virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582
Page seeded by OCA on Thu Mar 20 10:30:01 2008
