3w37
From Proteopedia
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| - | + | ==Sugar beet alpha-glucosidase with acarbose== | |
| - | === | + | <StructureSection load='3w37' size='340' side='right' caption='[[3w37]], [[Resolution|resolution]] 1.70Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3w37]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Beta_vulgaris Beta vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W37 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W37 FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACR:ALPHA-ACARBOSE'>ACR</scene>, <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w38|3w38]], [[3wel|3wel]], [[3wem|3wem]], [[3wen|3wen]], [[3weo|3weo]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-glucosidase Alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.20 3.2.1.20] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w37 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w37 RCSB], [http://www.ebi.ac.uk/pdbsum/3w37 PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sugar beet alpha-glucosidase (SBG), a member of glycoside hydrolase family 31, shows exceptional long-chain specificity, exhibiting higher kcat/Kms for longer maltooligosaccharides. However, its amino acid sequence is similar to other short-chain-specific alpha-glucosidases. To gain structural insights into the long-chain-substrate recognition of SBG, a crystal structure complex with the pseudotetrasaccharide, acarbose, was determined at 1.7 A resolution. The active site pocket of SBG was formed by a (beta/alpha)8 barrel domain and a long loop (the N-loop) bulging from the N-terminal domain similar to other related enzymes. Two residues, Phe236 and Asn237 in the N-loop were important for the long-chain specificity. Kinetic analysis of an Asn237 mutant enzyme and a previous study of a Phe236 mutant enzyme demonstrated that these residues create subsites +2 and +3. The structure also indicated that Phe236 and Asn237 guided the reducing end of long substrates to subdomain b2, which is an additional element inserted into the (beta/alpha)8 barrel domain. Subdomain b2 of SBG included Ser497, which was identified as the residue at subsite +4 by site-directed mutagenesis. | ||
| - | + | Molecular basis for the recognition of long-chain substrates by plant alpha-glucosidase.,Tagami T, Yamashita K, Okuyama M, Mori H, Yao M, Kimura A J Biol Chem. 2013 May 16. PMID:23687304<ref>PMID:23687304</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Alpha-glucosidase]] | [[Category: Alpha-glucosidase]] | ||
[[Category: Beta vulgaris]] | [[Category: Beta vulgaris]] | ||
Revision as of 06:26, 30 July 2014
Sugar beet alpha-glucosidase with acarbose
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