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OCA (Talk | contribs) (New page: ==A structural model of the active ribosome-bound membrane protein insertase YidC== <StructureSection load='4utq' size='340' side='right' caption='4utq, resolution 8.00&...) Next diff →
4utq is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
The integration of most membrane proteins into the cytoplasmic membrane of bacteria occurs co-translationally. The universally conserved YidC protein mediates this process either individually as a membrane protein insertase, or in concert with the SecY complex. Here, we present a structural model of YidC based on evolutionary co-variation analysis, lipid-versus-protein-exposure and molecular dynamics simulations. The model suggests a distinctive arrangement of the conserved five transmembrane domains and a helical hairpin between transmembrane segment 2 (TM2) and TM3 on the cytoplasmic membrane surface. The model was used for docking into a cryo-electron microscopy reconstruction of a translating YidC-ribosome complex carrying the YidC substrate FOc. This structure reveals how a single copy of YidC interacts with the ribosome at the ribosomal tunnel exit and identifies a site for membrane protein insertion at the YidC protein-lipid interface. Together, these data suggest a mechanism for the co-translational mode of YidC-mediated membrane protein insertion.
A structural model of the active ribosome-bound membrane protein insertase YidC.,Wickles S, Singharoy A, Andreani J, Seemayer S, Bischoff L, Berninghausen O, Soeding J, Schulten K, van der Sluis EO, Beckmann R Elife (Cambridge). 2014 Jul 10:e03035. doi: 10.7554/eLife.03035. PMID:25012291[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Wickles S, Singharoy A, Andreani J, Seemayer S, Bischoff L, Berninghausen O, Soeding J, Schulten K, van der Sluis EO, Beckmann R. A structural model of the active ribosome-bound membrane protein insertase YidC. Elife (Cambridge). 2014 Jul 10:e03035. doi: 10.7554/eLife.03035. PMID:25012291 doi:http://dx.doi.org/10.7554/eLife.03035
