1d6k
From Proteopedia
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| - | [[Image:1d6k.gif|left|200px]] | + | [[Image:1d6k.gif|left|200px]] |
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| - | '''NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX''' | + | {{Structure |
| + | |PDB= 1d6k |SIZE=350|CAPTION= <scene name='initialview01'>1d6k</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1D6K is a [ | + | 1D6K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6K OCA]. |
==Reference== | ==Reference== | ||
| - | The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition., Stoldt M, Wohnert J, Ohlenschlager O, Gorlach M, Brown LR, EMBO J. 1999 Nov 15;18(22):6508-21. PMID:[http:// | + | The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition., Stoldt M, Wohnert J, Ohlenschlager O, Gorlach M, Brown LR, EMBO J. 1999 Nov 15;18(22):6508-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10562563 10562563] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:33:36 2008'' |
Revision as of 08:33, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX
Overview
The structure of the complex between ribosomal protein L25 and a 37 nucleotide RNA molecule, which contains the E-loop and helix IV regions of the E-domain of Escherichia coli 5S rRNA, has been determined to an overall r.m.s. displacement of 1.08 A (backbone heavy atoms) by heteronuclear NMR spectroscopy (Protein Databank code 1d6k). The interacting molecular surfaces are bipartite for both the RNA and the protein. One side of the six-stranded beta-barrel of L25 recognizes the minor groove of the E-loop with very little change in the conformations of either the protein or the RNA and with the RNA-protein interactions occurring mainly along one strand of the E-loop duplex. This minor groove recognition module includes two parallel beta-strands of L25, a hitherto unknown RNA binding topology. Binding of the RNA also induces conversion of a flexible loop to an alpha-helix in L25, the N-terminal tip of which interacts with the widened major groove at the E-loop/helix IV junction of the RNA. The structure of the complex reveals that the E-domain RNA serves as a preformed docking partner, while the L25 protein has one preformed and one induced recognition module.
About this Structure
1D6K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition., Stoldt M, Wohnert J, Ohlenschlager O, Gorlach M, Brown LR, EMBO J. 1999 Nov 15;18(22):6508-21. PMID:10562563
Page seeded by OCA on Thu Mar 20 10:33:36 2008
