Journal:JBIC:26

Fluoride inhibition of Sporosarcina pasteurii urease: structure and thermodynamics

Stefano Benini, Michele Cianci, Luca Mazzei and Stefano Ciurli ^[1]

Molecular Tour
is a nickel-dependent enzyme (Ni(II) ions are shown as green spheres, α, β, and γ subunits are colored in darkmagenta, yellow, deeppink, respectively) and a virulence factor for ureolytic bacterial human pathogens, but it is also necessary to convert urea (see static image below), the most worldwide used fertiliser, into forms of nitrogen that can be taken up by crop plants.

A strategy to control the activity of urease for medical and agricultural applications is to use enzyme inhibitors. Fluoride is a known urease inhibitor, but the structural basis of its mode of inhibition are still undetermined. Here, kinetic studies on the fluoride-induced inhibition of urease from Sporosarcina pasteurii, a widespread and highly ureolytic soil bacterium, revealed a mixed competitive and uncompetitive mechanism. The pH-dependence of the inhibition constants, investigated in the 6.5-8.0 range, reveals a predominant uncompetitive mechanism that increases by increasing the pH, and a lesser competitive inhibition that increases by lowering the pH. Ten crystal structures of the enzyme were independently determined using five crystals of the and five crystals of the protein crystallised in the presence of fluoride. The analysis of these structures revealed the presence of , in terminal and bridging positions. The present study consistently supports an interaction of fluoride with the nickel centres in the urease active site in which one fluoride competitively binds to the Ni(II) ion proposed to coordinate urea in the initial step of the catalytic mechanism, while another fluoride uncompetitively substitutes the Ni(II)-bridging hydroxide, blocking its nucleophilic attack on urea.