4uqi
From Proteopedia
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uqi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uqi RCSB], [http://www.ebi.ac.uk/pdbsum/4uqi PDBsum]</span></td></tr> | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uqi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uqi RCSB], [http://www.ebi.ac.uk/pdbsum/4uqi PDBsum]</span></td></tr> | ||
<table> | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clathrin-mediated endocytosis (CME) is vital for the internalization of most cell-surface proteins. In CME, plasma membrane-binding clathrin adaptors recruit and polymerize clathrin to form clathrin-coated pits into which cargo is sorted. Assembly polypeptide 2 (AP2) is the most abundant adaptor and is pivotal to CME. Here, we determined a structure of AP2 that includes the clathrin-binding beta2 hinge and developed an AP2-dependent budding assay. Our findings suggest that an autoinhibitory mechanism prevents clathrin recruitment by cytosolic AP2. A large-scale conformational change driven by the plasma membrane phosphoinositide phosphatidylinositol 4,5-bisphosphate and cargo relieves this autoinhibition, triggering clathrin recruitment and hence clathrin-coated bud formation. This molecular switching mechanism can couple AP2's membrane recruitment to its key functions of cargo and clathrin binding. | ||
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| + | Clathrin adaptors. AP2 controls clathrin polymerization with a membrane-activated switch.,Kelly BT, Graham SC, Liska N, Dannhauser PN, Honing S, Ungewickell EJ, Owen DJ Science. 2014 Jul 25;345(6195):459-63. doi: 10.1126/science.1254836. PMID:25061211<ref>PMID:25061211</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 02:26, 7 August 2014
AP2 controls clathrin polymerization with a membrane-activated switch
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