1dcs

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[[Image:1dcs.gif|left|200px]]<br /><applet load="1dcs" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1dcs.gif|left|200px]]
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caption="1dcs, resolution 1.3&Aring;" />
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'''DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS'''<br />
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{{Structure
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|PDB= 1dcs |SIZE=350|CAPTION= <scene name='initialview01'>1dcs</scene>, resolution 1.3&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1DCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCS OCA].
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1DCS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCS OCA].
==Reference==
==Reference==
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Structure of a cephalosporin synthase., Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J, Andersson I, Nature. 1998 Aug 20;394(6695):805-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9723623 9723623]
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Structure of a cephalosporin synthase., Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J, Andersson I, Nature. 1998 Aug 20;394(6695):805-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9723623 9723623]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]
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[[Category: 2-oxoglutarate]]
[[Category: 2-oxoglutarate]]
[[Category: antibiotic biosynthesis]]
[[Category: antibiotic biosynthesis]]
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[[Category: antibiotics]]
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[[Category: antibiotic]]
[[Category: cephalosporin]]
[[Category: cephalosporin]]
[[Category: ferrous oxygenase]]
[[Category: ferrous oxygenase]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:36:25 2008''

Revision as of 08:36, 20 March 2008

Image:1dcs.gif


PDB ID 1dcs

Drag the structure with the mouse to rotate
, resolution 1.3Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS


Overview

Penicillins and cephalosporins are among the most widely used therapeutic agents. These antibiotics are produced from fermentation-derived materials as their chemical synthesis is not commercially viable. Unconventional steps in their biosynthesis are catalysed by Fe(II)-dependent oxidases/oxygenases; isopenicillin N synthase (IPNS) creates in one step the bicyclic nucleus of penicillins, and deacetoxycephalosporin C synthase (DAOCS) catalyses the expansion of the penicillin nucleus into the nucleus of cephalosporins. Both enzymes use dioxygen-derived ferryl intermediates in catalysis but, in contrast to IPNS, the ferryl form of DAOCS is produced by the oxidative splitting of a co-substrate, 2-oxoglutarate (alpha-ketoglutarate). This route of controlled ferryl formation and reaction is common to many mononuclear ferrous enzymes, which participate in a broader range of reactions than their well-characterized counterparts, the haem enzymes. Here we report the first crystal structure of a 2-oxoacid-dependent oxygenase. High-resolution structures for apo-DAOCS, the enzyme complexed with Fe(II), and with Fe(II) and 2-oxoglutarate, were obtained from merohedrally twinned crystals. Using a model based on these structures, we propose a mechanism for ferryl formation.

About this Structure

1DCS is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.

Reference

Structure of a cephalosporin synthase., Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J, Andersson I, Nature. 1998 Aug 20;394(6695):805-9. PMID:9723623

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