1dg6
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1dg6.gif|left|200px]] | + | [[Image:1dg6.gif|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STRUCTURE OF APO2L/TRAIL''' | + | {{Structure |
| + | |PDB= 1dg6 |SIZE=350|CAPTION= <scene name='initialview01'>1dg6</scene>, resolution 1.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF APO2L/TRAIL''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1DG6 is a [ | + | 1DG6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG6 OCA]. |
==Reference== | ==Reference== | ||
| - | A unique zinc-binding site revealed by a high-resolution X-ray structure of homotrimeric Apo2L/TRAIL., Hymowitz SG, O'Connell MP, Ultsch MH, Hurst A, Totpal K, Ashkenazi A, de Vos AM, Kelley RF, Biochemistry. 2000 Feb 1;39(4):633-40. PMID:[http:// | + | A unique zinc-binding site revealed by a high-resolution X-ray structure of homotrimeric Apo2L/TRAIL., Hymowitz SG, O'Connell MP, Ultsch MH, Hurst A, Totpal K, Ashkenazi A, de Vos AM, Kelley RF, Biochemistry. 2000 Feb 1;39(4):633-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10651627 10651627] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 28: | Line 37: | ||
[[Category: zinc-binding site]] | [[Category: zinc-binding site]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:38:06 2008'' |
Revision as of 08:38, 20 March 2008
| |||||||
| , resolution 1.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF APO2L/TRAIL
Contents |
Overview
Apoptosis-inducing ligand 2 (Apo2L, also called TRAIL), a member of the tumor necrosis factor (TNF) family, induces apoptosis in a variety of human tumor cell lines but not in normal cells [Wiley, S. R., Schooley, K., Smolak, P. J., Din, W. S., Huang, C.-P., Nicholl, J. K., Sutherland, G. R., Smith, T. D., Rauch, C., Smith, C. A., and Goodwin, R. G. (1995) Immunity 3, 673-682; Pitti, R. M., Marsters, S. A., Ruppert, S., Donahue, C. J., Moore, A., and Ashkenazi, A. (1996) J. Biol. Chem. 271, 12687-12690]. Here we describe the structure of Apo2L at 1.3 A resolution and use alanine-scanning mutagenesis to map the receptor contact regions. The structure reveals a homotrimeric protein that resembles TNF with receptor-binding epitopes at the interface between monomers. A zinc ion is buried at the trimer interface, coordinated by the single cysteine residue of each monomer. The zinc ion is required for maintaining the native structure and stability and, hence, the biological activity of Apo2L. This is the first example of metal-dependent oligomerization and function of a cytokine.
Disease
Known disease associated with this structure: Squamous cell carcinoma, head and neck OMIM:[603612]
About this Structure
1DG6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A unique zinc-binding site revealed by a high-resolution X-ray structure of homotrimeric Apo2L/TRAIL., Hymowitz SG, O'Connell MP, Ultsch MH, Hurst A, Totpal K, Ashkenazi A, de Vos AM, Kelley RF, Biochemistry. 2000 Feb 1;39(4):633-40. PMID:10651627
Page seeded by OCA on Thu Mar 20 10:38:06 2008
Categories: Homo sapiens | Single protein | ConnelL, M P.O. | Hymowitz, S G. | Kelley, R F. | Ultsch, M H. | Vos, A M.de. | CL | ZN | Cytokine | Tnf | Trimer | Zinc-binding site
