1dgp
From Proteopedia
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| - | [[Image:1dgp.jpg|left|200px]] | + | [[Image:1dgp.jpg|left|200px]] |
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| - | '''ARISTOLOCHENE SYNTHASE FARNESOL COMPLEX''' | + | {{Structure |
| + | |PDB= 1dgp |SIZE=350|CAPTION= <scene name='initialview01'>1dgp</scene>, resolution 2.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FOH:FARNESOL'>FOH</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Aristolochene_synthase Aristolochene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.9 4.2.3.9] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ARISTOLOCHENE SYNTHASE FARNESOL COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DGP is a [ | + | 1DGP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_roqueforti Penicillium roqueforti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DGP OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti., Caruthers JM, Kang I, Rynkiewicz MJ, Cane DE, Christianson DW, J Biol Chem. 2000 Aug 18;275(33):25533-9. PMID:[http:// | + | Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti., Caruthers JM, Kang I, Rynkiewicz MJ, Cane DE, Christianson DW, J Biol Chem. 2000 Aug 18;275(33):25533-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10825154 10825154] |
[[Category: Aristolochene synthase]] | [[Category: Aristolochene synthase]] | ||
[[Category: Penicillium roqueforti]] | [[Category: Penicillium roqueforti]] | ||
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[[Category: sesquiterpene cyclase]] | [[Category: sesquiterpene cyclase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:38:19 2008'' |
Revision as of 08:38, 20 March 2008
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| , resolution 2.8Å | |||||||
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| Ligands: | |||||||
| Activity: | Aristolochene synthase, with EC number 4.2.3.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ARISTOLOCHENE SYNTHASE FARNESOL COMPLEX
Overview
The 2.5-A resolution crystal structure of recombinant aristolochene synthase from the blue cheese mold, Penicillium roqueforti, is the first of a fungal terpenoid cyclase. The structure of the enzyme reveals active site features that participate in the cyclization of the universal sesquiterpene cyclase substrate, farnesyl diphosphate, to form the bicyclic hydrocarbon aristolochene. Metal-triggered carbocation formation initiates the cyclization cascade, which proceeds through multiple complex intermediates to yield one exclusive structural and stereochemical isomer of aristolochene. Structural homology of this fungal cyclase with plant and bacterial terpenoid cyclases, despite minimal amino acid sequence identity, suggests divergence from a common, primordial ancestor in the evolution of terpene biosynthesis.
About this Structure
1DGP is a Single protein structure of sequence from Penicillium roqueforti. Full crystallographic information is available from OCA.
Reference
Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti., Caruthers JM, Kang I, Rynkiewicz MJ, Cane DE, Christianson DW, J Biol Chem. 2000 Aug 18;275(33):25533-9. PMID:10825154
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