1dj3
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1dj3.jpg|left|200px]] | + | [[Image:1dj3.jpg|left|200px]] |
| - | + | ||
| - | '''STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM AND ARABIDOPSIS THALIANA''' | + | {{Structure |
| + | |PDB= 1dj3 |SIZE=350|CAPTION= <scene name='initialview01'>1dj3</scene>, resolution 3.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM AND ARABIDOPSIS THALIANA''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1DJ3 is a [ | + | 1DJ3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJ3 OCA]. |
==Reference== | ==Reference== | ||
| - | Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana., Prade L, Cowan-Jacob SW, Chemla P, Potter S, Ward E, Fonne-Pfister R, J Mol Biol. 2000 Feb 18;296(2):569-77. PMID:[http:// | + | Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana., Prade L, Cowan-Jacob SW, Chemla P, Potter S, Ward E, Fonne-Pfister R, J Mol Biol. 2000 Feb 18;296(2):569-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10669609 10669609] |
[[Category: Adenylosuccinate synthase]] | [[Category: Adenylosuccinate synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: gdp]] | [[Category: gdp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:39:18 2008'' |
Revision as of 08:39, 20 March 2008
| |||||||
| , resolution 3.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Adenylosuccinate synthase, with EC number 6.3.4.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM AND ARABIDOPSIS THALIANA
Overview
Catalyzing the first step in the de novo synthesis of adenylmonophosphate, adenylosuccinate synthetase (AdSS) is a known target for herbicides and antibiotics. We have purified and crystallized recombinant AdSS from Arabidopsis thaliana and Tritium aestivum, expressed in Escherichia coli. The structures of A. thaliana and T. aestivum AdSS in complex with GDP were solved at 2.9 A and 3.0 A resolution, respectively. Comparison with the known structures from E. coli reveals that the overall fold is very similar to that of the E. coli protein. The longer N terminus in the plant sequences is at the same place as the longer C terminus of the E. coli sequence in the 3D structure. The GDP-binding sites have one additional hydrogen-bonding partner, which is a plausible explanation for the lower K(m) value. Due to its special position, this partner may also enable GTP to initiate a conformational change, which was, in E. coli AdSS, exclusively activated by ligands at the IMP-binding site. The dimer interfaces show up to six hydrogen bonds and six salt-bridges more than in the E. coli structure, although the contact areas have approximately the same size.
About this Structure
1DJ3 is a Single protein structure of sequence from Triticum aestivum. Full crystallographic information is available from OCA.
Reference
Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana., Prade L, Cowan-Jacob SW, Chemla P, Potter S, Ward E, Fonne-Pfister R, J Mol Biol. 2000 Feb 18;296(2):569-77. PMID:10669609
Page seeded by OCA on Thu Mar 20 10:39:18 2008
