Adenosine dimethyltransferase

Function

Adenosine dimethyltransferase (KsgA) dimethylates two adjacent adenines at the C-terminal of the 16S rRNA in the small 30S ribosomal subunit, thus modifying it. KsgA catalyzes the dimethylation of adenines 1518-1519 using S-adenosyl-methionine (SAM) as the methyl donor and producing S-adenosyl-homocysteine (SAH).

Disease

Relevance

KsgA confers resistance to the antibiotic kasugomycin.

Structural highlights

3D structures of adenosine dimethyltransferase

Updated on 13-August-2014

1qyr – EcKsgA – Escherichia coli

3tpz – EcKsgA (mutant)
3ftc, 3ftd – AaKsgA – Aquifex aeolicus
3fut, 3fuv – TtKsgA – Thermus thermophilus
3tqs – KsgA – Coxiella burnetii
3uzu – KsgA – Burkholderia pseudomallei
4jxj – KsgA – Rickettsia bellii

Adenosine dimethyltransferase complex

3fte – AaKsgA + RNA
3ftf – AaKsgA + RNA + SAH
3fuu, 3fuw, 3fux – TtKsgA + adenosine derivative
3grr – MjKsgA (mutant) + SAH – Methanocaldococcus jannaschii
3gru – MjKsgA (mutant) + AMP
3grv – MjKsgA (mutant) + adenosine
3gry – MjKsgA (mutant) + SAM
3r9x – AaKsgA + GDPNP + RNA + GTPase
4adv – EcKsgA + 30S ribosomal subunit – Cryo-EM

References