1dt0
From Proteopedia
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| - | [[Image:1dt0.gif|left|200px]] | + | [[Image:1dt0.gif|left|200px]] |
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| - | '''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS''' | + | {{Structure |
| + | |PDB= 1dt0 |SIZE=350|CAPTION= <scene name='initialview01'>1dt0</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE:FE (III) ION'>FE</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DT0 is a [ | + | 1DT0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT0 OCA]. |
==Reference== | ==Reference== | ||
| - | Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:[http:// | + | Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11053832 11053832] |
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
[[Category: FE]] | [[Category: FE]] | ||
| - | [[Category: pseudomonas | + | [[Category: pseudomonas ovali]] |
[[Category: superoxide dismutase]] | [[Category: superoxide dismutase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:43:50 2008'' |
Revision as of 08:43, 20 March 2008
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| , resolution 2.1Å | |||||||
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| Ligands: | |||||||
| Activity: | Superoxide dismutase, with EC number 1.15.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS
Overview
The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.
About this Structure
1DT0 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Reference
Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:11053832
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