1dvm

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[[Image:1dvm.gif|left|200px]]<br /><applet load="1dvm" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1dvm.gif|left|200px]]
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caption="1dvm, resolution 2.40&Aring;" />
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'''ACTIVE FORM OF HUMAN PAI-1'''<br />
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{{Structure
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|PDB= 1dvm |SIZE=350|CAPTION= <scene name='initialview01'>1dvm</scene>, resolution 2.40&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY=
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|GENE= UMBILICAL VEIN ENDOTHELIUM LIBRARY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''ACTIVE FORM OF HUMAN PAI-1'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1DVM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVM OCA].
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1DVM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVM OCA].
==Reference==
==Reference==
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Structures of active and latent PAI-1: a possible stabilizing role for chloride ions., Stout TJ, Graham H, Buckley DI, Matthews DJ, Biochemistry. 2000 Jul 25;39(29):8460-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10913251 10913251]
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Structures of active and latent PAI-1: a possible stabilizing role for chloride ions., Stout TJ, Graham H, Buckley DI, Matthews DJ, Biochemistry. 2000 Jul 25;39(29):8460-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10913251 10913251]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: serpin]]
[[Category: serpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:45:05 2008''

Revision as of 08:45, 20 March 2008

Image:1dvm.gif


PDB ID 1dvm

Drag the structure with the mouse to rotate
, resolution 2.40Å
Ligands:
Gene: UMBILICAL VEIN ENDOTHELIUM LIBRARY (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



ACTIVE FORM OF HUMAN PAI-1


Contents

Overview

Serpins exhibit a range of physiological roles and can contribute to certain disease states dependent on their various conformations. Understanding the mechanisms of the large-scale conformational reorganizations of serpins may lead to a better understanding of their roles in various cardiovascular diseases. We have studied the serpin, plasminogen activator inhibitor 1 (PAI-1), in both the active and the latent state and found that anionic halide ions may play a role in the active-to-latent structural transition. Crystallographic analysis of a stable mutant form of active PAI-1 identified an anion-binding site between the central beta-sheet and a small surface domain. A chloride ion was modeled in this site, and its identity was confirmed by soaking crystals in a bromide-containing solution and calculating a crystallographic difference map. The anion thus located forms a 4-fold ligated linchpin that tethers the surface domain to the central beta-sheet into which the reactive center loop must insert during the active-to-latent transition. Timecourse experiments measuring active PAI-1 stability in the presence of various halide ions showed a clear trend for stabilization of the active form with F(-) > Cl(-) > Br(-) >> I(-). We propose that the "stickiness" of this pin (i.e., the electronegativity of the anion) contributes to the energetics of the active-to-latent transition in the PAI-1 serpin.

Disease

Known diseases associated with this structure: Hemorrhagic diathesis due to PAI1 deficiency OMIM:[173360], Thrombophilia due to excessive plasminogen activator inhibitor OMIM:[173360]

About this Structure

1DVM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structures of active and latent PAI-1: a possible stabilizing role for chloride ions., Stout TJ, Graham H, Buckley DI, Matthews DJ, Biochemistry. 2000 Jul 25;39(29):8460-9. PMID:10913251

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