1dxj

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[[Image:1dxj.gif|left|200px]]<br /><applet load="1dxj" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1dxj.gif|left|200px]]
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caption="1dxj, resolution 1.8&Aring;" />
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'''STRUCTURE OF THE CHITINASE FROM JACK BEAN'''<br />
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{{Structure
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|PDB= 1dxj |SIZE=350|CAPTION= <scene name='initialview01'>1dxj</scene>, resolution 1.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]
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|GENE=
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}}
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'''STRUCTURE OF THE CHITINASE FROM JACK BEAN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1DXJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DXJ OCA].
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1DXJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DXJ OCA].
==Reference==
==Reference==
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Structure of jack bean chitinase., Hahn M, Hennig M, Schlesier B, Hohne W, Acta Crystallogr D Biol Crystallogr. 2000 Sep;56(Pt 9):1096-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10957628 10957628]
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Structure of jack bean chitinase., Hahn M, Hennig M, Schlesier B, Hohne W, Acta Crystallogr D Biol Crystallogr. 2000 Sep;56(Pt 9):1096-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10957628 10957628]
[[Category: Canavalia ensiformis]]
[[Category: Canavalia ensiformis]]
[[Category: Chitinase]]
[[Category: Chitinase]]
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[[Category: hydrolase]]
[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:46:01 2008''

Revision as of 08:46, 20 March 2008

Image:1dxj.gif


PDB ID 1dxj

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands:
Activity: Chitinase, with EC number 3.2.1.14
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE CHITINASE FROM JACK BEAN


Overview

The structure of jack bean chitinase was solved at 1.8 A resolution by molecular replacement. It is an alpha-helical protein with three disulfide bridges. The active site is related in structure to animal and viral lysozymes. However, unlike in lysozyme, the architecture of the active site suggests a single-step cleavage. According to this mechanism, Glu68 is the proton donor and Glu90 assists in the reaction by moving towards the substrate and recruiting a water molecule that acts as the nucleophile. In this model, a water molecule was found in contact with Glu90 O(epsilon1) and Thr119 O(gamma) at a distance of 3.0 and 2.8 A, respectively. The model is in accordance with the observed inversion mechanism.

About this Structure

1DXJ is a Single protein structure of sequence from Canavalia ensiformis. Full crystallographic information is available from OCA.

Reference

Structure of jack bean chitinase., Hahn M, Hennig M, Schlesier B, Hohne W, Acta Crystallogr D Biol Crystallogr. 2000 Sep;56(Pt 9):1096-9. PMID:10957628

Page seeded by OCA on Thu Mar 20 10:46:01 2008

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