1dyt

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[[Image:1dyt.gif|left|200px]]<br /><applet load="1dyt" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1dyt.gif|left|200px]]
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caption="1dyt, resolution 1.75&Aring;" />
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'''X-RAY CRYSTAL STRUCTURE OF ECP (RNASE 3) AT 1.75 A'''<br />
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{{Structure
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|PDB= 1dyt |SIZE=350|CAPTION= <scene name='initialview01'>1dyt</scene>, resolution 1.75&Aring;
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|SITE= <scene name='pdbsite=ASA:Potential+Active+Site+Chain+A'>ASA</scene>
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene> and <scene name='pdbligand=CIT:CITRIC ACID'>CIT</scene>
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|ACTIVITY=
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|GENE= RNASE3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''X-RAY CRYSTAL STRUCTURE OF ECP (RNASE 3) AT 1.75 A'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1DYT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FE:'>FE</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=ASA:Potential+Active+Site+Chain+A'>ASA</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DYT OCA].
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1DYT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DYT OCA].
==Reference==
==Reference==
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Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 A resolution., Mallorqui-Fernandez G, Pous J, Peracaula R, Aymami J, Maeda T, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Ruth FX, Coll M, J Mol Biol. 2000 Jul 28;300(5):1297-307. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10903870 10903870]
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Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 A resolution., Mallorqui-Fernandez G, Pous J, Peracaula R, Aymami J, Maeda T, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Ruth FX, Coll M, J Mol Biol. 2000 Jul 28;300(5):1297-307. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10903870 10903870]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rnase 3]]
[[Category: rnase 3]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:46:41 2008''

Revision as of 08:46, 20 March 2008

Image:1dyt.gif


PDB ID 1dyt

Drag the structure with the mouse to rotate
, resolution 1.75Å
Sites:
Ligands: and
Gene: RNASE3 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



X-RAY CRYSTAL STRUCTURE OF ECP (RNASE 3) AT 1.75 A


Overview

Eosinophil cationic protein (ECP; RNase 3) is a human ribonuclease found only in eosinophil leukocytes that belongs to the RNase A superfamily. This enzyme is bactericidal, helminthotoxic and cytotoxic to mammalian cells and tissues. The protein has been cloned, heterologously overexpressed, purified and crystallized. Its crystal structure has been determined and refined using data up to 1. 75 A resolution. The molecule displays the alpha+beta folding topology typical for members of the ribonuclease A superfamily. The catalytic active site residues are conserved with respect to other ribonucleases of the superfamily but some differences appear at substrate recognition subsites, which may account, in part, for the low catalytic activity. Most strikingly, 19 surface-located arginine residues confer a strong basic character to the protein. The high concentration of positive charges and the particular orientation of the side-chains of these residues may also be related to the low activity of ECP as a ribonuclease and provides an explanation for its unique cytotoxic role through cell membrane disruption.

About this Structure

1DYT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 A resolution., Mallorqui-Fernandez G, Pous J, Peracaula R, Aymami J, Maeda T, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Ruth FX, Coll M, J Mol Biol. 2000 Jul 28;300(5):1297-307. PMID:10903870

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